Detecting and monitoring endophytic colonization by Pseudomonas putida BP25 in black pepper (Piper nigrum L.) using quantitative real-time PCR.
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Title |
Detecting and monitoring endophytic colonization by Pseudomonas putida BP25 in black pepper (Piper nigrum L.) using quantitative real-time PCR.
Monitoring endophytic colonization in black pepper |
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Creator |
Agisha V N, Eapen S J, Suseelabhai R
Kumar A |
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Subject |
black pepper, endophytic colonization, Pseudomonas putida, real- time PCR
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Description |
Not Available
A quantitative real-time PCR assay was developed to quantify Pseudomonas putida BP25, anantagonistic endophyte against a broad range of pathogens in black pepper such as Phytophthoracapsici, Colletotrichum gloeosporioides, Rhizoctonia solani, Gibberella moniliformis, Athelia rolfsii and aplant parasitic nematode, Radopholus similis. The real-time PCR primers were designed based onthe16S rRNA sequences of P. putida strains and specificity of the primers was confirmed. Thedetection limit of the assay was found to be 1 pg. The assay detected and quantified the bacterialcolonization in the roots at weekly intervals after inoculation. The P. putida DNA was quantifiedto be 0.4 ng in roots corresponding to 5.4 log10 CFU g-1 at 7th and 14th day after inoculation (DAI).A decline in endophyte population was observed during 21st and 28th DAI and the DNAconcentration ranged from 3.7-4.6 pg corresponding to 3.4-3.5 log10 CFU g-1 of root. Noamplification could be obtained in stem and leaf samples. The newly developed real-time PCRcould be useful for detection, quantification and monitoring of endophytic P. putida BP25 indifferent plant tissues. Not Available |
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Date |
2021-08-07T18:05:31Z
2021-08-07T18:05:31Z 2017-06-26 |
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Type |
Research Paper
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Identifier |
Agisha, V. N., Eapen, S. J., Bhai, R. S., & Kumar, A. (2017). Detecting and monitoring endophytic colonization by Pseudomonas putida BP25 in black pepper (Piper nigrum L.) using quantitative real-time PCR. Journal of Spices and Aromatic Crops, 26(1), 01–07. https://doi.org/10.25081/josac.2017.v26.i1.812
0971-3328 http://krishi.icar.gov.in/jspui/handle/123456789/53617 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Indian Society for Spices
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