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Gene expression analysis in drought tolerant and susceptible black pepper (Piper nigrum L.) in response to water deficit stress

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Title Gene expression analysis in drought tolerant and susceptible black pepper (Piper nigrum L.) in response to water deficit stress
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Creator Johnson George K
Neema Malik
Vijesh Kumar IP
Krishnamurthy Ks
 
Subject Gene expression
drought tolerant
black pepper
Piper nigrum L.
water deficit stress
 
Description Not Available
Drought or water deficit stress is one of the main environmental stresses affecting plants, resulting in reduced productivity and crop loss. Black pepper, a major spice cultivated across the globe, is drought sensitive and water stress often results in plant death. The present study compared the difference in physiological parameters: relative water content (RWC) and cell membrane leakage, and also analyzed the differential expression of 11 drought responsive genes in drought tolerant and drought sensitive black pepper genotypes. Tolerant black pepper genotype exhibited significantly higher RWC and lower cell membrane leakage 10 days after stress induction than the sensitive genotype. The relative expressions of the 11 selected drought responsive genes were normalized against ubiquitin and RNA-binding protein which was identified as the most stable reference genes in black pepper under the present experimental condition using the RefFinder software. Dehydrin showed the highest transcript accumulation in both the black pepper genotypes under drought stress condition and the relative expression of the gene was higher in the tolerant genotype compared to the susceptible. Similar pattern of higher relative expression was also observed in the stress responsive gene, osmotin. The membrane protein aquaporin and the transcription factor bZIP were relatively down-regulated in the tolerant genotype. The differential expression of these important drought responsive genes in tolerant genotype of black pepper indicates its further usefulness in developing varieties with improved water stress tolerance.
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Date 2021-08-12T06:24:18Z
2021-08-12T06:24:18Z
2017-04
 
Type Research Paper
 
Identifier Kokkat, Johnson & Malik, Neema & Kumar, Vijesh & Ks, Krishnamurthy. (2017). Gene expression analysis in drought tolerant and susceptible black pepper (Piper nigrum L.) in response to water deficit stress. Acta Physiologiae Plantarum. 39. 10.1007/s11738-017-2398-5.
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http://krishi.icar.gov.in/jspui/handle/123456789/55255
 
Language English
 
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Publisher Not Available