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Enhancing shelf life of Trichoderma harzianum by conidial storage in sterile deionized water

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Title Enhancing shelf life of Trichoderma harzianum by conidial storage in sterile deionized water
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Creator Suseela Bhai R
Anandaraj M
 
Subject biological control
black pepper
conidial suspension
cryo-preservatives
foot rot
liquid formulation
Phytophthora capsici
Trichoderma harzianum
 
Description Not Available
In this study T. harzianum conidial suspension was made in different concentrations of cryoprotectants viz., glycerol, glucose, DMSO and deionized distilled water and stored at two different temperatures viz., 25± 1oC and 28-32ºC for more than 720 days for evaluating the shelflife in order to develop a ready to use liquid carrier formulation. The results showed that submerged conidia formulation in cryoprotectants like glycerol, glucose and DMSO preservedthe viability for 75 days only when compared to the formulation in sterile deionized water which at 28-32°C retained the viability for 480 days with a viable count of loge= 6.671 (3.2 × 106) and for more than 720 days with a viable count of loge=5.54 (2 × 105) and in 480 days at 25±1°C with avaiable count of loge=6.3911 (6.5 × 106) and with a viable count of loge =5.40 (2 × 105) for more than720 days. The viability was tested in vitro by recovery in TSM and effectiveness was tested in vivo by drenching the suspension on plants and challenge inoculating with Phytophthora capsici afterseven days. Challenge inoculation of black pepper plants with P. capsici after drenching withdiluted stored suspension gave a disease reduction of 66.67%. Hence, sterile deionized water can be used as a liquid storage medium for the long term storage of T. harzianum culture without loosing the viability and effectiveness of the spores
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Date 2021-08-13T08:32:27Z
2021-08-13T08:32:27Z
2014-12-24
 
Type Research Paper
 
Identifier Not available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/56276
 
Language English
 
Relation Not Available;
 
Publisher Not Available