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An efficient protein extraction method for proteomic analysis of black pepper (Piper nigrum L.) and generation of protein map using nano LC-LTQ Orbitrap mass spectrometry.

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Title An efficient protein extraction method for proteomic analysis of black pepper (Piper nigrum L.) and generation of protein map using nano LC-LTQ Orbitrap mass spectrometry.
Not Available
 
Creator P Umadevi
M Anandaraj
 
Subject 2DE
extraction methods
recalcitrant plant
proteogenomics
 
Description Not Available
Information on black pepper proteins can accelerate the basic understanding of crop response to various biotic, abiotic stresses as well as the crop improvement programmes. Here, we present the protocols for total leaf protein extraction from this non model, recalcitrant plant species P. nigrum after evaluating 5 different standard methods. The efficiency and suitability of the methods were evaluated using important parameters such as protein quantity and quality, solubility in IEF compatible buffer and streak free results, number of spots in 2DE gel. Modified lysis buffer and phenol method were found to be applicable methods for protein extraction from black pepper. Among two applicable methods, the phenol protocol is time-consuming, demanding the toxic phenol. Hence, we propose the modified lysis buffer method as superior, rapid method with no polyphenol contamination for total leaf protein extraction. This is a very first report which evaluated different methods and optimized the standards for the analysis of black pepper leaf proteome. Successful identification of randomly selected spots by nano LC-LTQ Orbitrap proves its compatibility to high throughput MS analysis which leads to the generation of leaf protein map, the first of its kind.
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Date 2021-09-06T05:57:01Z
2021-09-06T05:57:01Z
2015-01
 
Type Research Paper
 
Identifier Palaniyandi, Umadevi & Muthuswamy, Anandaraj. (2015). An efficient protein extraction method for proteomic analysis of black pepper (Piper nigrum L.) and generation of protein map using nano LC-LTQ Orbitrap mass spectrometry. 8. 500-507.
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http://krishi.icar.gov.in/jspui/handle/123456789/61376
 
Language English
 
Relation Not Available;
 
Publisher ICAR- Indian institute of spices research,Kozhikode, Kerala India