Record Details

Indirect and direct somatic embryogenesis from aerial stem explants of ginger (Zingiber officinale Rosc.)

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Indirect and direct somatic embryogenesis from aerial stem explants of ginger (Zingiber officinale Rosc.)
Not Available
 
Creator A K Lincy
A B Remasree
B Sasikumar
 
Subject Aerial stem
Zingiber
histology
somatic embryogenesis
 
Description Not Available
Protocols for direct and indirect somatic embryogenesis from aerial stem explants of ginger have been developed. Aerial stem explants of two ginger varieties were cultured on different concentrations of 2,4-D induced callus. An in vitro aerial stem produced hard,nodular and yellowish callus (Type I) and an in planta aerial stem gave rise to soft, sticky callus with pale white color (Type II). The proliferated Type II calli were subject to stress for 40–60 days without subculturing. The desiccated calli produced white friable calli
which turned embryogenic and then produced somatic embryos in a medium containing 2mg L–1 benzyl amino purine. The mature, club-shaped somatic embryos were germinated on a medium containing benzyl amino purine and a– naphthalene acetic acid in different concentrations. Type I callus of neither variety turned embryogenic but produced roots in all the cultures. Direct somatic embryogenesis was observed from the in planta aerial stem and leaf base explants with the use of thidiazuron alone or in combination with
indole, 3-butyric acid. Histological studies revealed that the somatic embryos in gingerhave a distinct single layered epidermis, scutellum, coleoptile, shoot apex and root apex
Not Available
 
Date 2021-09-14T09:48:32Z
2021-09-14T09:48:32Z
2009
 
Type Research Paper
 
Identifier Lincy, Adinkudik & B, Rema shree. (2009). Indirect and direct somatic embryogenesis from aerial stem explants of ginger (Zingiber officinale Rosc.). Acta Botanica Croatica (acta@biol.pmf.hr); Vol.68 No.1.. 68.
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/62012
 
Language English
 
Relation Not Available;
 
Publisher ICAR- Indian institute of spices research,Kozhikode, Kerala India