Differential induction of chitinase in Piper colubrinum in response to inoculation with Phytophthora capsici, the cause of foot rot in black pepper.
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Title |
Differential induction of chitinase in Piper colubrinum in response to inoculation with Phytophthora capsici, the cause of foot rot in black pepper.
Not Available |
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Creator |
R Sandeep Varma
George, Johnson K S Balaji V A Parthasarathy |
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Subject |
Chitinase assay
Phytophthora capsici Piper Reverse transcription |
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Description |
Not Available
Plant chitinases have been of particular interest since they are known to be induced upon pathogen invasion. Inoculation of Piper colubrinum leaves with the foot rot fungus, Phytophthora capsici leads to increase in chitinase activity. A marked increase in chitinase activity in the inoculated leaves was observed, with the maximum activity after 60 h of inoculation and gradually decreased thereafter. Older leaves showed more chitinase activity than young leaves. The level of chitinase in black pepper (Piper nigrum L.) upon inoculation was found to be substantially high when compared to P. colubrinum. RT-PCR using chitinase specific primers revealed differential accumulation of mRNA in P. colubrinum leaves inoculated with P. capsici. However, hyphal extension assays revealed no obvious differences in the ability of the protein extracts to inhibit growth of P. capsici in vitro. Not Available |
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Date |
2021-09-14T09:45:21Z
2021-09-14T09:45:21Z 2009-07 |
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Type |
Research Paper
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Identifier |
Sandeep Varma R, Johnson George K, Balaji S, Parthasarathy VA. Differential induction of chitinase in Piper colubrinum in response to inoculation with Phytophthora capsici, the cause of foot rot in black pepper. Saudi J Biol Sci. 2009 Jul;16(1):11-6. doi: 10.1016/j.sjbs.2009.07.002. PMID: 23961037; PMCID: PMC3730965.
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/62009 |
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Language |
English
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Relation |
Not Available;
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Publisher |
ICAR- Indian institute of spices research,Kozhikode, Kerala India
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