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ISSR markers for genetic diversity analysis in spices

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Title ISSR markers for genetic diversity analysis in spices
Not Available
 
Creator K Johnson George
V R Sandeep
G Ganga
P Utpala
K V Saji
Parthasarathy V A
 
Subject ISSR markers
genetic diversity
spices
 
Description Not Available
Inter-simple sequence repeat (IISR) is a multilocus DNA marker increasingly being used for DNA typing of plants. Three non-anchored primers were tested for assessing the suitability of its use in genetic diversity analysis of spices germplasm. Optimum annealing temperature for different primers varied from 42 to 63°C depending on the base sequences of the primers.All the primers were successfully used in amplifying inter-microsatellite regions of small cardamom, large cardamom, different species of vanilla and Pipe. Band profiles obtained with ISSR primers were found to be highly polymorphic and reproducible. The ISSR primer (GACA)4 yielded reproducible and consistent DNA banding pattern in Piper and was found to be useful in distinguishing the seven species selected for the study. It is suggested that ISSR markers, in addition to being simple and time efficient, allow rapid identification of polymorphisms within spices germplasm
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Date 2021-09-14T09:42:13Z
2021-09-14T09:42:13Z
2006
 
Type Research Paper
 
Identifier Johnson George, K., Sandeep V. R., Ganga, G., Utpala, P., Saji, K. V. and V. A Parthasarathy. (2006) ISSR markers for genetic diversity analysis in spices. An appraisal. Indian Journal of HorticuHure 63: 302-304.
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http://krishi.icar.gov.in/jspui/handle/123456789/62002
 
Language English
 
Relation Not Available;
 
Publisher ICAR- Indian institute of spices research,Kozhikode, Kerala India