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First report of genome size and ploidy of the underutilized leguminous tuber crop Yam Bean (Pachyrhizus erosus and P. tuberosus) by flow cytometry

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Title First report of genome size and ploidy of the underutilized leguminous tuber crop Yam Bean (Pachyrhizus erosus and P. tuberosus) by flow cytometry
Not Available
 
Creator Kalidas Pati
Fangning Zhang
Jacqueline Batley
 
Subject DNA content, flow cytometry, genome size, Pachyrhizus spp, ploidy level, underutilized crop
 
Description Not Available
Cytological understanding is an important parameter to understand the genetic architecture of yam
bean. The ploidy level and genome size of two cultivated species of yam bean (Pachyrhizus erosus
and P. tuberosus)were estimated using flow cytometric analysis of young leaf tissue, with propidium
iodide as a fluorescent dye. Six genotypes of P. erosus and three genotypes of P. tuberosus were
analysed. Rice (Oryza sativa cv. Nipponbare) and Mung bean (Vigna radiata cv Berken) were
used as internal reference standards. Variation of 2C nuclear DNA content among the six P. erousus
lines was 4.18%, ranging from 1.17 to 1.22 pg, whereas only 1.8% variation was observed among the
three P. tuberosus lines, which ranged from 1.07 to 1.09 pg. Moreover, it was found that the nuclear
DNA content of P. tuberosus was lower than that of P. erosus. The result of the flow cytometric analysis
showed that all the species were diploid (2n = 2x) and coefficient of variation (CV%) of all the
accessions of the two species was less than 3.5%. This is the first report of ploidy analysis and genome
size estimation of the leguminous underutilized tuber crop yam bean using flow cytometry.
This result will be helpful for yam bean genome sequencing and crop improvement programmes
Not Available
 
Date 2022-02-02T13:32:03Z
2022-02-02T13:32:03Z
2019-05-22
 
Type Research Paper
 
Identifier Not Available
1479-2621
http://krishi.icar.gov.in/jspui/handle/123456789/69403
 
Language English
 
Relation Not Available;
 
Publisher Not Available