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Genetic diversity analysis in Indian mustard (Brassica juncea L. Czern & Coss) using RAPD markers

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Title Genetic diversity analysis in Indian mustard (Brassica juncea L. Czern & Coss) using RAPD markers
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Creator Mahla HR
Jambhulkar SJ
Sharma R
Mohapatra T
Mehta HR
Chaudhary BR
 
Subject Brassica juncea
 
Description Not Available
Genetic Diversity among 45 genotypes of Brassica juncea including released varieties, mutants, advanced breeding material and single plant selections was estimated using 37 decamer primers of 80 initially screened. A total of 205 amplicons were obtained with an average of 5.54 bands/primer, of these 162 were found to be polymorphic and the level of polymorphism was 79.02%. The number of bands generated was more primer dependent than the genotype and ranged from 1-19. Though all the genotypes were discriminated in the present study still the identification of such a large number of genotypes was not possible with limited number of primers. A few of the primers generated variety specific bands for a limited number of genotypes. Jaccard’s pairwise similarity coefficient values for 45 genotypes were calculated and the range of genetic similarity was found to be 0.60 to 0.97 with the average of 0.791+_ 0.002. A dendrogram was generated by UPGMA cluster analysis based on Jaccard’s similarity coefficients, which showed poor grouping of genotypes since the similarity among the varieties and groups were comparable. Genotypes were grouped as per their pedigree or origin.
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Date 2021-08-06T10:47:07Z
2021-08-06T10:47:07Z
2015-01-01
 
Type Research Paper
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/53450
 
Language English
 
Relation Not Available;
 
Publisher Not Available