Record Details

Development of sub-tropically adapted diverse provitamin-A rich maize inbreds through marker-assisted pedigree selection, their characterization and utilization in hybrid breeding

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Development of sub-tropically adapted diverse provitamin-A rich maize inbreds through marker-assisted pedigree selection, their characterization and utilization in hybrid breeding
Not Available
 
Creator Hriipulou Duo
Firoz Hossain
Vignesh Muthusamy
Rajkumar U. Zunjare
Rajat Goswami
Gulab Chand
Subhra J. Mishra
Rashmi Chhabra
Munegowda M. Gowda
Saikat Pal
Aanchal Baveja
Jayant S. Bhat
Mehar C. Kamboj
Bhupender Kumar
John J. Amalraj
Rajesh Khulbe
Bhukya Prakash
C. N. Neeraja
Sujay Rakshit
Om P. Yadav
 
Subject Sub-tropically adapted diverse provitamin-A rich maize inbreds
Marker-assisted pedigree selection
Hybrid breeding
 
Description Not Available
Malnutrition has emerged as one of the major health problems worldwide. Traditional yellow maize has low provitamin-A (proA) content and its genetic base in proA biofortification breeding program of subtropics is extremely narrow. To diversify the proA rich germplasm, 10 elite low proA inbreds were crossed with a proA rich donor (HP702-22) having mutant crtRB1 gene. The F2 populations derived from these crosses were genotyped using InDel marker specific to crtRB1. Severe marker segregation distortion was observed. Seventeen crtRB1 inbreds developed through marker-assisted pedigree breeding and seven inbreds generated using marker-assisted backcross breeding were characterized using 77 SSRs. Wide variation in gene diversity (0.08 to 0.79) and dissimilarity coefficient (0.28 to 0.84) was observed. The inbreds were grouped into three major clusters depicting the existing genetic diversity. The crtRB1-based inbreds possessed high β-carotene (BC: 8.72μg/g), β-cryptox- anthin (BCX: 4.58μg/g) and proA (11.01μg/g), while it was 2.35μg/g, 1.24μg/g and 2.97μg/g in checks, respectively. Based on their genetic relationships, 15 newly developed crtRB1- based inbreds were crossed with five testers (having crtRB1 gene) using line × tester mating design. 75 experimental hybrids with crtRB1 gene were evaluated over three locations.
These experimental hybrids possessed higher BC (8.02μg/g), BCX (4.69μg/g), proA (10.37μg/g) compared to traditional hybrids used as check (BC: 2.36 μg/g, BCX: 1.53μg/g, proA: 3.13μg/g). Environment and genotypes × environment interaction had minor effects on proA content. Both additive and dominance gene action were significant for proA.
Not Available
 
Date 2021-08-03T05:37:39Z
2021-08-03T05:37:39Z
2021-02-04
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/52446
 
Language English
 
Relation Not Available;
 
Publisher PLOS ONE