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Deciphering the mechanisms of endophyte-mediated biofortification of Fe and Zn in wheat

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Title Deciphering the mechanisms of endophyte-mediated biofortification of Fe and Zn in wheat
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Creator Singh D.
N. Geat
M.V.S. Rajawat
M. Mahajan
R. Prasanna
S. Singh
R. Kaushik
R.N. Singh
K. Kumar
A.K. Saxena
 
Subject Anatomical features
Gene expression
Root exudates
Organic acids
TaZIP
TEM
 
Description Not Available
An investigation was carried out to understand the mechanism(s) underlying enhanced Fe or Zn uptake
in low Fe–Zn accumulator wheat genotype 4HPYT-414, due to inoculation of siderophore-producing and zinc-solubilizing endophytes—Arthrobacter sulfonivorans DS-68 and Arthrobacter sp. DS-179. Root anatomical features, using transmission electron microscopy (TEM), qualitative and quantitative aspects of production of organic acids and sugars in root exudates, and expression of TaZIP genes were analysed to relate to endophyte-mediated higher concentrations of Fe and Zn in the roots and shoots of wheat plants. TEM studies revealed that the endodermis, cortical region, root hair extension, xylem and xylem vessels, pericycle and vascular bundles were more pronounced and thicker in inoculated treatments, as compared to control.
The organic acid profile of root exudates revealed five types of organic acids, with citric acid being predominant. Inoculation of A. sulfonivorans and Arthrobacter sp. brought
about 5- and eightfold increases in the amounts of acids,respectively, as compared to control, particularly citric
acid, succinic acid and acetic acid. Among the four TaZIP genes targeted, expression was achieved only for TaZIP3 and TaZIP7 genes, which showed 1–2 fold increases in the inoculated treatments. The results clearly indicated that the endophyte-mediated overexpression of TaZIP3 and TaZIP7 genes in roots and shoots, and the observed anatomical and exudate changes were acting synergistically in facilitating higher Fe and Zn translocation in roots and shoots.
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Date 2021-08-06T06:05:57Z
2021-08-06T06:05:57Z
2017-05-16
 
Type Article
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/53353
 
Language English
 
Relation Not Available;
 
Publisher Not Available