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Detection of Salmonella from clinical samples of dogs by PCR

Indian Agricultural Research Journals

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Title Detection of Salmonella from clinical samples of dogs by PCR
 
Creator Verma, A K
Sinha, D K
Singh, B R
 
Subject Dog; PCR; Salmonella species
 
Description In present cross sectional study, faecal swabs and blood samples were collected from 250 dogs of Bareilly city, Uttar Pradesh, India and examined for Salmonella spp. using standard culture method and polymerase chain reaction (PCR) using primers Cohen-1 and Cohen-2. Salmonella were isolated from 1 faecal and 2 blood clots samples. Isolates from faeces were characterized as S. Weltevreden [3, 10; r; z6] and that from blood as S. Typhimurium [4, 5, 12; i; 1, 2). Whereas an amplicon of 496 bp was amplified from 6 faecal and 4 blood clot samples. As known positive controls, viz. DNA extract of S. Typhi and cell lysate of S. Typhimurium, also yielded similar amplicons. The relative sensitivity, relative specificity and accuracy of PCR considering isolation as gold standard was found 100%, 97.17% and 97.20%, respectively. The PCR was found more rapid and sensitive than traditional culture method of isolation from clinical samples which further required biochemical and serological testing for confirmation.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2011-05-20
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/6228
 
Source The Indian Journal of Animal Sciences; Vol 81, No 6 (2011)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/6228/2515
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences