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Isolation and electropherotyping of equine rotaviruses from diarrhoeic foals in India

Indian Agricultural Research Journals

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Title Isolation and electropherotyping of equine rotaviruses from diarrhoeic foals in India
 
Creator Gulati, Baldev R
Malik, Praveen
Kumar, Rajender
 
Subject Equine rotavirus; Foal diarrhoea; Isolation; RNA-PAGE
 
Description Equine rotavirus is one of the major etiological agents associated with diarrhoea in foals. The rotavirus was detected in 4 of 50 (8%) diarrhoeic foals below 2 months of age in a farm near Hisar (Haryana), using RNA-PAGE and group A rotavirus ELISA kit. Rotaviruses were isolated from all the positive samples in MA104 cell lines alter 6-8 blind passages in serum-free MEM medium supplemented with 2 µg/ml af 11 typsin.The RNA profiles of the rotavirus isolates were compared with refrence bovine group A rotaviruses (NCDV, B223, CR129 and CK156). The electrophorectic profile of the equine rotavirus isolates From the outbreak in June 2003 (FRV2 and FRV3) differed from those, of the july outbreak (FRV25 and FRV28) in migration of RNA segment 5. confirming that at least 2 distinct strains of equine rotaviruses are prevalent in the region. This is the first report of isolation and electropherotyping of rotaviruses from diarrhoeic foals in India.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Date 2005-07-05
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/8149
 
Source The Indian Journal of Animal Sciences; Vol 75, No 7 (2005)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/8149/3180
 
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