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Effect of Electron Beam Irradiation in Combination with Other Treatments on Shrimp Allergen, Tropomyosin

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Title Effect of Electron Beam Irradiation in Combination with Other Treatments on Shrimp Allergen, Tropomyosin
Not Available
 
Creator Laly, S.J.
Jeyakumari, A.
Kuppa Sivasankara Sarma
Kaushlesh Pansingh Rawat
Shaik Abdul Khader
Sankar T. V.
Panda, S. K.
 
Subject Electron beam irradiation
IgE activity
Immunoblotting
Shrimp tropomyosin
 
Description Not Available
A hurdle technology-based approach for the reduction in immunogenicity
of shrimp major allergen, tropomyosin was investigated by combining electron beam
irradiation at 5 kGy and boiling, autoclaving, trypsin and chymotrypsin treatments
along with peeled raw shrimp as control. Shrimp extracts were prepared from the
irradiated sample and evaluated SDS PAGE profile, IgE binding ability and immunogenicity of tropomyosin using pooled sera of shrimp sensitive individuals. Electron
beam irradiation resulted in significant (p < 0.05) decrease in the IgE activity in the
case of all treatments. The SDS PAGE analysis showed the presence of tropomyosin
in all the treatments except autoclaving in combination with or without electron beam
irradiation. A pronounced reduction in allergenicity was observed in the treatment of autoclaving in combination with electron beam irradiation. The combined application of autoclaving and electron beam irradiation can be utilized for the preparation of hypoallergic shrimp.
Not Available
 
Date 2023-06-06T04:03:43Z
2023-06-06T04:03:43Z
2022-08-10
 
Type Book chapter
 
Identifier Laly, S.J., Jeyakumari, A., Kuppa Sivasankara Sarma, Kaushlesh Pansingh Rawat, Shaik Abdul Khader, Sankar T. V. and Panda, S. K. (2022) Effect of Electron Beam Irradiation in Combination with Other Treatments on Shrimp Allergen, Tropomyosin. In: Lama, T., Burman, D., Mandal, U.K., Sarangi, S.K., Sen, H. (eds) Transforming Coastal Zone for Sustainable Food and Income Security. Springer, Cham. https://doi.org/10.1007/978-3-030-95618-9_44. 577-586.
978-3-030-95618-9
http://krishi.icar.gov.in/jspui/handle/123456789/78042
 
Language English
 
Relation Not Available;
 
Publisher Springer