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Non-diarrhoeic pigs as source of highly virulent and multidrug-resistant non-typhoidal Salmonella

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Title Non-diarrhoeic pigs as source of highly virulent and multidrug-resistant non-typhoidal Salmonella
Not Available
 
Creator Nagappa Karabasanavar
Sivaraman, G. K.
Satheesha S. P.
Archana S Nair
Ardhra Vijayan
Vineeth Rajan
Girish P.S.
 
Subject Bacteria
Serotype
PFGE
Virulence
Resistance
Genes
 
Description Not Available
Food-producing animals act as reservoirs of non-typhoidal Salmonella (NTS) serovars with potential food safety and public health implications. The present cross-sectional study aimed at determining the prevalence of Salmonella serotypes in non-diarrhoeic pigs and characterizing the isolates using molecular tools. Salmonella isolates (n = 22) recovered from faecal samples of 194 randomly selected pigs were characterized for virulence and antimicrobial resistance and subtyped using XbaI-PFGE. The prevalence of Salmonella in apparently healthy non-diarrhoeic pigs was 11.3% (95%CI, 4.3–19.5%), with S. Weltevreden (81.8%) and S. Enteritidis (18.2%) being the serotypes detected. Salmonella isolates harboured virulence genes such as invA (100%), stn (100%), spvR/spvC (86.3%) and fimA (22.7%). Phenotypically, isolates showed sensitivity to chloramphenicol, levofloxacin and ciprofloxacin and resistance to tetracycline and ampicillin (100%), streptomycin (86.4%), amoxicillin-clavulanate (63.6%), cefotaxime (22.7%) and ceftriaxone (9.1%). Notably, 18.2% isolates were multidrug-resistant (≥ 3 antimicrobial class) with multiple antimicrobial resistance (MAR) index of 0.56–0.67 (18.2%), 0.44 (45.5%), 0.33 (31.8%) and 0.22 (4.5%). Genotypically, isolates carried various antibiotic resistance genes: ESBL (blaTEM and blaOXA), aminoglycoside (strA, strB and aadA1), sulphonamide (sul1, sul2 and dfrA1), tetracycline (tetA and tetB) and plasmid AmpC beta-lactamase (ACC, FOX, MOX, DHA, CIT and EBC). The present investigation emphasizes the epidemiological significance of PFGE typing in the detection of emerging strains of highly virulent and multidrug-resistant S. Weltevreden and S. Enteritidis in non-diarrhoeic pigs that pose serious public health implications in the pork supply chain environment. More extensive longitudinal study is warranted to provide epidemiological links between environmental reservoirs and animal and human infections in piggery settings.
Not Available
 
Date 2023-06-06T04:05:11Z
2023-06-06T04:05:11Z
2022-02-07
 
Type Research Paper
 
Identifier Nagappa Karabasanavar, Sivaraman, ,G. K., Satheesha S. P., Archana S Nair, Ardhra Vijayan,Vineeth Rajan, Girish P.S. (2022) Non-diarrhoeanic pigs as source of highly virulent and multidrug resistant non-typhoid Salmonella, Brazilian Journal of Microbiology. 53: 1039–1049.
1678-4405
http://krishi.icar.gov.in/jspui/handle/123456789/78044
 
Language English
 
Relation Not Available;
 
Publisher Springer