The anti-peptide relaxin antibodies for monitoring the well-being of the fetus in pregnant bitches.
KRISHI: Publication and Data Inventory Repository
View Archive InfoField | Value | |
Title |
The anti-peptide relaxin antibodies for monitoring the well-being of the fetus in pregnant bitches.
Not Available |
|
Creator |
Shruthi. N.
Indhu M. A. M.Shende A. M. Pawde Praveen Singh S. K. Bhure |
|
Subject |
Canine
Relaxin peptide Pregnant bitch |
|
Description |
Not Available
Pregnancy management is difficult in canines and there is a lack of methodology which would allow monitoring embryonic well-being relaxin (Rlx) is being reported for use in pregnancy diagnosis in different species. Here, we evaluated seven anti-Rlx peptide antibodies for detection of well-being of the fetus in bitches. Peptides were synthesized using solid phase peptide synthesis chemistry and the hyper-immune sera raised in chicken against peptides. In sandwich ELISA, the chicken anti-Rlx P4 as a capture and rabbit anti-prorelaxin as detection antibody, gave better results in terms of differentiating the pregnant from non-pregnant bitches. A 35 canine serum samples (21 non-pregnant, 11 pregnant and 3 males) were screened. Among the 11 pregnant, six delivered normally and the rest of the bitches aborted a few days after the serum collection with one or two dead fetuses. Among the 11 pregnant serum samples, 4 showed the absorbance above the cutoff value set for pregnancy, which delivered healthy puppies and five bitches showed the absorbance below the set cutoff value aborted after a few days of blood collection with one or two dead fetuses. The specificity of the assay was found to be 90.47 % and the sensitivity of the assay for detecting the well-being of the fetus is 82%. The serum samples collected from those bitches having problems related to normal whelping, had shown absorbance below the set cut-off value. The results of the study indicate that relaxin is a useful marker, specifically for monitoring the pregnancy and tells about the well-being of the fetus. Not Available |
|
Date |
2023-06-07T03:52:09Z
2023-06-07T03:52:09Z 2018-01-01 |
|
Type |
Research Paper
|
|
Identifier |
Not Available
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/78074 |
|
Language |
English
|
|
Relation |
Not Available;
|
|
Publisher |
NIScPR
|
|