Record Details

The anti-peptide relaxin antibodies for monitoring the well-being of the fetus in pregnant bitches.

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title The anti-peptide relaxin antibodies for monitoring the well-being of the fetus in pregnant bitches.
Not Available
 
Creator Shruthi. N.
Indhu M.
A. M.Shende
A. M. Pawde
Praveen Singh
S. K. Bhure
 
Subject Canine
Relaxin peptide
Pregnant bitch
 
Description Not Available
Pregnancy management is difficult in canines and there is a lack of methodology which would allow monitoring embryonic well-being relaxin (Rlx) is being reported for use in pregnancy diagnosis in different species. Here, we evaluated seven anti-Rlx peptide antibodies for detection of well-being of the fetus in bitches. Peptides were synthesized using solid phase peptide synthesis chemistry and the hyper-immune sera raised in chicken against peptides. In sandwich ELISA, the chicken anti-Rlx P4 as a capture and rabbit anti-prorelaxin as detection antibody, gave better results in terms of differentiating the pregnant from non-pregnant bitches. A 35 canine serum samples (21 non-pregnant, 11 pregnant and 3 males) were screened. Among the 11 pregnant, six delivered normally and the rest of the bitches aborted a few days after the serum collection with one or two dead fetuses. Among the 11 pregnant serum samples, 4 showed the absorbance above the cutoff value set for pregnancy, which delivered healthy puppies and five bitches showed the absorbance below the set cutoff value aborted after a few days of blood collection with one or two dead fetuses. The specificity of the assay was found to be 90.47 % and the sensitivity of the assay for detecting the well-being of the fetus is 82%. The serum samples collected from those bitches having problems related to normal whelping, had shown absorbance below the set cut-off value. The results of the study indicate that relaxin is a useful marker, specifically for monitoring the pregnancy and tells about the well-being of the fetus.
Not Available
 
Date 2023-06-07T03:52:09Z
2023-06-07T03:52:09Z
2018-01-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/78074
 
Language English
 
Relation Not Available;
 
Publisher NIScPR