Record Details

Comparative study of heparin-binding proteins profile of Murrah buffalo (Bubalus bubalis) semen

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Comparative study of heparin-binding proteins profile of Murrah buffalo (Bubalus bubalis) semen
Not Available
 
Creator S. S. Ramteke
A. M. Shende
J. S. Rajoriya
N. C. Barik
Megha Pande
S. K. Bhure
G. K. Das
S. K. Ghosh
J. K. Prasad
 
Subject buffalo bull
heparin-binding proteins
semen
seminal plasma
 
Description Not Available
Aim: The experiment was conducted to study the total seminal plasma protein (TSPP) and heparin-binding proteins (HBPs) in relation to initial semen quality of buffalo bull. Materials and Methods: Semen from two Murrah buffalo bulls (bull no. 605 and 790) with mass motility of ≥3+ were used for the study and categorized into three groups (Group I- Mass motility 3+, Group II- Mass motility 4+ and Group III- Mass motility 5+). Seminal plasma from semen was separated by centrifugation. HBPs was isolated and purified from heparin-agarose affinity column by modified elution buffer. TSPP and isolated HBPs concentration was estimated by Lowry’s method. The purified HBPs were resolved on Sodium dodecyl sulfate polyacrylamide gel
electrophoresis to check the protein profile of two bulls.
Results: The mean values of TSPP concentrations in bull no. 605 and 790 in Group I, II and III were 30.64±0.12, 31.66±0.09, 32.53±0.19 and 28.51±0.09, 29.49±0.15, 30.45±0.17 mg/mL, respectively. The mean values of HBPs concentrations in bull no. 605 and 790 in Group I, II and III were 3.11±0.07, 3.32±0.06, 3.46±0.08 and 2.51±0.08, 2.91±0.05, 3.10±0.03 mg/mL, respectively. Both the values of TSPP and HBPs were significantly higher (p
Not Available
 
Date 2023-06-07T08:21:05Z
2023-06-07T08:21:05Z
2014-09-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/78085
 
Language English
 
Relation Not Available;
 
Publisher Not Available