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Humoral immune response in Asian seabass vaccinated with inactivated and recombinant viral nervous necrosis vaccine

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Title Humoral immune response in Asian seabass vaccinated with inactivated and recombinant viral nervous necrosis vaccine
Not Available
 
Creator M. Makesh
N. Venkata Satyanarayana
K. Muddukrishnaiah
Sujeet Kumar
G. Thiagarajan
Ashok Kumar Jangam
R. Subburaj
M. Kailasam
K.K. Vijayan
 
Subject Viral nervous necrosis
Red-spotted grouper NNV
Inactivated vaccine
Recombinant vaccine
Maternal antibodies
 
Description Not Available
Viral nervous necrosis is a serious viral disease of marine and brackishwater finfish hatcheries. The red-spotted
grouper nervous necrosis virus (RGNNV) is the most prevalent genotype among fishes in tropical and temperate
regions. RGNNV was propagated in SSN-1 cell line and inactivated using BEI. The capsid protein of RGNNV was
cloned and expressed in Escherichia coli and purified. Inactivated and recombinant VNN vaccines were produced
by emulsifying the antigen with commercial adjuvant and administered to Asian seabass, Lates calcarifer, fingerlings
and broodstock intraperitoneally. The immune response was assessed at regular intervals by indirect
ELISA. The maternal antibodies in one-day post-hatch larvae obtained from the vaccinated and control broodstock
were assessed by indirect ELISA. The larvae were subjected to challenge with virulent RGNNV and the
relative per cent survival (RPS) was estimated. The efficacy of the vaccine was also assessed under field conditions.
The recombinant vaccine produced significantly higher specific antibody levels than the inactivated
vaccine in fingerlings and broodstock and the immune response was dose-dependent. Recombinant vaccine at 50
and 100 μg/fish produced significantly higher antibody levels and immune response could be observed as early
as one-week post vaccination. Vaccinated broodstock produced larvae with maternal antibodies and had higher
RPS when challenged at 18 days post-hatch. The vaccine produced a significant immune response even under
field conditions. The vaccine can be used to vaccinate broodstock to reduce loss due to VNN in early larval stages
Not Available
 
Date 2023-06-09T04:30:38Z
2023-06-09T04:30:38Z
2023-02-18
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/78168
 
Language English
 
Relation Not Available;
 
Publisher Not Available