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MexAB-OprM efflux pump of Pseudomonas aeruginosa offers tolerance to carvacrol: A herbal antimicrobial agent.

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Title MexAB-OprM efflux pump of Pseudomonas aeruginosa offers tolerance to carvacrol: A herbal antimicrobial agent.
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Creator Vadhana P, Singh BR, Pesingi PK, Bhardwaj M, Singh SV, Kumawat M, Sinha DK, Gandham RK.
 
Subject MexAB-OprM Efflux Pump of Pseudomonas aeruginosa Offers Resistance to Carvacrol: A Herbal Antimicrobial Agent
 
Description Not Available
Carvacrol is a herbal antimicrobial agent with in vitro activity against several bacterial pathogens. However, multidrug resistant strains of Pseudomonas aeruginosa are resistant to herbal antimicrobial compounds including carvacrol. Resistance of P. aeruginosa to carvacrol is not well studied. This study was aimed to identify the gene(s) associated with carvacrol resistance, thus to understand its mechanisms in P. aeruginosa. A herbal drug resistant strain was isolated from a hospital environment. Carvacrol sensitive mutant was generated using transposon mutagenesis. The inactivated gene in the mutant was identified as mexA, which is part of the mexAB-oprM operon. Inactivation of the mexA gene resulted in a >31-fold reduction in MIC of carvacrol, whereas a >80-fold reduction was observed in the presence of drug efflux inhibitor phenylalanine-arginine β-naphthylamide (PAβN). The parental herbal-resistant strain was completely killed within 3 h of incubation in the presence of carvacrol and PAβN. The mexA inactivation did not affect the resistance to other herbal compounds used. The results demonstrate that resistance to carvacrol in P. aeruginosa is mediated by the MexAB-OprM efflux pump.
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Date 2023-06-14T04:58:00Z
2023-06-14T04:58:00Z
2019-11-01
 
Type Article
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/78312
 
Language English
 
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Publisher Not Available