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Cryoprotection of Humanin-like Peptides in Seminal Plasma for Ejaculated Spermatozoa of Crossbred Bulls

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Title Cryoprotection of Humanin-like Peptides in Seminal Plasma for Ejaculated Spermatozoa of Crossbred Bulls
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Creator Pande, Megha; Ghosh, S.K.; Tyagi, S.; Katiyar, R.; Srivastava, N.; Karikalan, M.; Kumar, S.; Krishnappa, K.; Sirohi, A.S.; Sarika; Mitra, A.
 
Subject CROSSBRED BULLS; FRIESWAL; HUMANIN; OXIDATIVE STRESS; SEMEN CRYOPRESERVATION
 
Description Not Available
Cryopreservation process negatively affects spermatozoa functions. Humanin, a small polypeptide encoded in the mitochondrial genome, is well known for its role in cell survival. OBJECTIVE: To quantify the endogenous levels of humanin in seminal plasma of crossbred Frieswal bulls and to study its role in cryoprotection. The presence of humanin in bull spermatozoa was also investigated. MATERIALS AND METHODS: A total of 40 semen samples were separated into two groups based on the initial progressive motility (IPM): Good (IPM ≥70%) and Poor ( IPM ≤50%) groups; and/or based on the post-thaw motility (PTM): Freezable (PTM≥50%) and Non-freezable (PTM < 50%) groups. Humanin concentration in seminal plasma (SP-HN) was quantified using ELISA. RESULTS: SP-HN concentration ranged from undetectable to 67.6 pg/mL with a median level of 35.2 pg/mL. SP-HN level was significantly higher in the good quality semen group than in the poor quality semen group (p
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Date 2023-06-14T04:41:32Z
2023-06-14T04:41:32Z
2022-09-01
 
Type Research Paper
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/78288
 
Language English
 
Relation Not Available;
 
Publisher Cryoletters