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Determination of the seminal leucocytary profile in cattle and buffalo semen using the Leishman, and Papanicolaou staining protocols

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Title Determination of the seminal leucocytary profile in cattle and buffalo semen using the Leishman, and Papanicolaou staining protocols
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Creator Anju Kujur, N. Srivastava, Aswini Sivan G, Shashikant Gupta,Nancy Jasrotia, G Singh4, SK Ghosh
 
Subject Leucocytary profile, Leishman stain, Papanicolaou stain
 
Description Not Available
Despite indications that seminal leucocytes can alter spermatozoa quality, investigations on the
qualitative and quantitative parameters of such cells in bull semen, as well as methods for determining
them are scarce. Therefore, the objective of this study was to assess the concentration of leucocytes in
different breeds and age groups using the staining methods viz. Leishman and Papanicolaou stain. Semen
ejaculates from two indigenous breeds (Sahiwal, Murrah) and one cross breed named Vrindavani were
randomly collected. For the studies on effects of age, the ejaculates were divided into 2 groups, namely
Group I, ejaculates from bulls less than 8 years of age (5 bulls x 6 ejaculates) and Group II, ejaculates
from bulls more than 8 years of age (5 x 6 x 2 groups = 60 samples). All the samples are stained with
Leishman and Papanicolaou stain for leucocytary counts. For the studies on effect of breed data was
divided into 6 ejaculates x 3 bulls x 3 breeds (n=54). The concentration of leucocytes comprising mostly
macrophages were found and was shown to be significantly higher in the Sahiwal breed (3.05±0.18) than
other breeds and in Group II, more than 8 years old bulls (3.16±0.16) with Leishman stain. The study
finds Leishman staining procedure better in terms of ease of doing and results obtained for leucocyte
count than the Papanicolaou staining protocol.
Not Available
 
Date 2023-06-14T04:39:57Z
2023-06-14T04:39:57Z
2022-10-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/78285
 
Language English
 
Relation Not Available;
 
Publisher The Pharma Innovation Journal