Occurrence, pathogroup distribution and virulence genotypes of Escherichia coli from fresh seafood
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Title |
Occurrence, pathogroup distribution and virulence genotypes of Escherichia coli from fresh seafood
Not Available |
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Creator |
Sreepriya Prakasan
Manjusha Lekshmi Parvathi Ammini Amjad K.Balange Binaya Bhusan Nayak Sanath H. Kumar |
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Subject |
Seafood
Escherichia coli Pathogroup Fecal contamination Risk assessment Serotype |
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Description |
Not Available
Seafood contamination with pathogenic Escherichia coli is a significant public health threat in developing countries. In this study, the prevalence of different pathogroups of E. coli was investigated in fresh seafood. A total of 78 samples comprising of finfish (41) and shellfish (37) were analyzed using multiple selective-enrichment-isolation protocols. From all samples, 639 E. coli were isolated and tested for the presence of pathogroup-specific virulence-associated genes by polymerase chain reaction (PCR). Based on the distribution of virulence genes, 118 isolates belonged to enterohemorrhagic E. coli (EHEC) group, 25 to enteropathogenic E. coli (EPEC), 20 to enterotoxigenic E. coli (ETEC), 16 to enteroaggregative E. coli (EAEC) and 1 to enteroinvasive E. coli (EIEC). Fifty-five samples of seafood, 29 finfish and 26 shellfish, were found contaminated with pathogenic E. coli. Among different enrichment broth-selective agar combinations used for the recovery of pathogenic E. coli from seafood, enrichment in Tryptone Phosphate (TP) broth followed by isolation on sorbitol MacConkey (SMAC) agar performed superior to others. The study highlights the importance of using selective isolation and genotyping protocols to identify human pathogenic E. coli in seafood in regions with extensive anthropogenic contamination. Not Available |
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Date |
2023-06-23T03:44:07Z
2023-06-23T03:44:07Z 2022-03 |
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Type |
Research Paper
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Identifier |
Sreepriya Prakasan, Manjusha Lekshmi, Parvathi Ammini, Amjad K.Balange, Binaya Bhusan Nayak, Sanath H. Kumar (2022) Occurrence, pathogroup distribution and virulence genotypes of Escherichia coli from fresh seafood. 133: 108669
1873-7129 http://krishi.icar.gov.in/jspui/handle/123456789/78874 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Elsevier
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