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Occurrence, pathogroup distribution and virulence genotypes of Escherichia coli from fresh seafood

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Title Occurrence, pathogroup distribution and virulence genotypes of Escherichia coli from fresh seafood
Not Available
 
Creator Sreepriya Prakasan
Manjusha Lekshmi
Parvathi Ammini
Amjad K.Balange
Binaya Bhusan Nayak
Sanath H. Kumar
 
Subject Seafood
Escherichia coli
Pathogroup
Fecal contamination
Risk assessment
Serotype
 
Description Not Available
Seafood contamination with pathogenic Escherichia coli is a significant public health threat in developing countries. In this study, the prevalence of different pathogroups of E. coli was investigated in fresh seafood. A total of 78 samples comprising of finfish (41) and shellfish (37) were analyzed using multiple selective-enrichment-isolation protocols. From all samples, 639 E. coli were isolated and tested for the presence of pathogroup-specific virulence-associated genes by polymerase chain reaction (PCR). Based on the distribution of virulence genes, 118 isolates belonged to enterohemorrhagic E. coli (EHEC) group, 25 to enteropathogenic E. coli (EPEC), 20 to enterotoxigenic E. coli (ETEC), 16 to enteroaggregative E. coli (EAEC) and 1 to enteroinvasive E. coli (EIEC). Fifty-five samples of seafood, 29 finfish and 26 shellfish, were found contaminated with pathogenic E. coli. Among different enrichment broth-selective agar combinations used for the recovery of pathogenic E. coli from seafood, enrichment in Tryptone Phosphate (TP) broth followed by isolation on sorbitol MacConkey (SMAC) agar performed superior to others. The study highlights the importance of using selective isolation and genotyping protocols to identify human pathogenic E. coli in seafood in regions with extensive anthropogenic contamination.
Not Available
 
Date 2023-06-23T03:44:07Z
2023-06-23T03:44:07Z
2022-03
 
Type Research Paper
 
Identifier Sreepriya Prakasan, Manjusha Lekshmi, Parvathi Ammini, Amjad K.Balange, Binaya Bhusan Nayak, Sanath H. Kumar (2022) Occurrence, pathogroup distribution and virulence genotypes of Escherichia coli from fresh seafood. 133: 108669
1873-7129
http://krishi.icar.gov.in/jspui/handle/123456789/78874
 
Language English
 
Relation Not Available;
 
Publisher Elsevier