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Cationic microparticle [poly(D,L - lactide - co - glycolide)] - coated DNA vaccination induces a long - term immune response against foot and mouth disease in guinea pigs

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Title Cationic microparticle [poly(D,L - lactide - co - glycolide)] - coated DNA vaccination induces a long - term immune response against foot and mouth disease in guinea pigs
Not Available
 
Creator Kotla S. Reddy
Brabhi R. Rashmi
Hosur J. Dechamma
Susarla Gopalakrishna
N. Banumathi
Veluvarthy V. S. Suryanarayana
Golla R. Reddy
 
Subject Foot-and-mouth disease
guinea pigs
Cationic microparticle
 
Description Not Available
Background: Foot and mouth disease (FMD) can be controlled by regular vaccination and restriction of the movement of infected animals in the endemic countries. Although presently used, tissue culture inactivated vaccine gives protection, it has several limitations, including a short duration of immunity. DNA vaccine delivered through microparticles could comprise an alternative approach to conventional vaccine when aiming to circumvent these limitations.

Methods: We constructed the expression plasmid (pVAC-1D) containing 1D gene FMD virus serotype Asia 1. Poly(D,L-lactide-co-glycolide) (PLG) microparticles were prepared and coated with the pVAC-1D plasmid. Guinea pigs were vaccinated with PLG-coated and naked DNA vaccine constructs intramuscularly. The humoral response was measured by an enzyme-linked immunosorbent assay (ELISA) and the serum neutralization test (SNT). Analysis of the persistence and the expression of pVAC-1D plasmid construct was carried out by quantitative polymerase chain reaction (qPCR).

Results: The humoral response lasted for 1 year, as measured by ELISA and SNT. Analysis of the persistence and the expression of pVAC-1D plasmid construct by qPCR has shown that pVAC-1D expression was seen for a longer duration compared to the naked DNA vaccine. Microparticles coated plasmid DNA-injected guinea pigs were protected when challenged with FMD virus.

Conclusions: The present study has shown that the delivery of plasmid coated on cationic PLG microparticles enhance the duration of immunity of the DNA vaccine constructs.
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Date 2023-07-05T05:50:32Z
2023-07-05T05:50:32Z
2012-03-21
 
Type Research Paper
 
Identifier Reddy KS, Rashmi BR, Dechamma HJ, Gopalakrishna S, Banumathi N, Suryanarayana VV, Reddy GR. Cationic microparticle [poly(D,L - lactide - co - glycolide)] - coated DNA vaccination induces a long - term immune response against foot and mouth disease in guinea pigs. J Gene Med. 2012 May;14(5):348 - 52. doi: 10.1002/jgm.2622. PMID: 22438260.
PMID: 22438260
http://krishi.icar.gov.in/jspui/handle/123456789/79613
 
Language English
 
Relation Not Available;
 
Publisher Wiley Online Library