Screening of bacmids for recombination
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Title |
Screening of bacmids for recombination
Not Available |
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Creator |
Vishweshwar Kumar Ganji
Sampath Kontham Mallesh Pottabathula Dechamma HJ |
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Subject |
BACMID
PCR TANSPOSITION |
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Description |
Not Available
In molecular biology, we most often handle with bacmid when working with baculovirus expression system. The cloning involves a two-step process and the recombinant bacmids are screened by bluewhite lac operon system, white colonies are thought to be positive for transposition. But in our study with PCR based approach, we found that not all the white colonies are recombinants. Hence, it is necessary to be cautious while screening for recombinant bacmids and may be it is good too screen by two-way approach, by blue-white lac operon system followed by PCR approach using one bacmid specific and one gene specific primers. Not Available |
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Date |
2023-07-05T05:53:22Z
2023-07-05T05:53:22Z 2018-01-01 |
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Type |
Article
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Identifier |
Not Available
ISSN (E): 2277- 7695 http://krishi.icar.gov.in/jspui/handle/123456789/79617 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Not Available
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