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Screening of bacmids for recombination

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Title Screening of bacmids for recombination
Not Available
 
Creator Vishweshwar Kumar Ganji
Sampath Kontham
Mallesh Pottabathula
Dechamma HJ
 
Subject BACMID
PCR
TANSPOSITION
 
Description Not Available
In molecular biology, we most often handle with bacmid when working with baculovirus expression
system. The cloning involves a two-step process and the recombinant bacmids are screened by bluewhite lac operon system, white colonies are thought to be positive for transposition. But in our study with
PCR based approach, we found that not all the white colonies are recombinants. Hence, it is necessary to
be cautious while screening for recombinant bacmids and may be it is good too screen by two-way
approach, by blue-white lac operon system followed by PCR approach using one bacmid specific and one
gene specific primers.
Not Available
 
Date 2023-07-05T05:53:22Z
2023-07-05T05:53:22Z
2018-01-01
 
Type Article
 
Identifier Not Available
ISSN (E): 2277- 7695
http://krishi.icar.gov.in/jspui/handle/123456789/79617
 
Language English
 
Relation Not Available;
 
Publisher Not Available