Record Details


Field	Value

Title	Effect of betaine and raffinose on post-thaw semen parameters and fertility in black pigmented Kadaknath chicken

Creator	Shanmugam M.

Subject	Semen

Description	Not Available BACKGROUND: Kadaknath is an important indigenous chicken with black pigmentation and cryopreserved semen reputably had low fertility. OBJECTIVE: The aim of this study was to evaluate the effects of betaine and raffinose in semen extenders on post thaw semen parameters and fertility. MATERIALS AND METHODS: Semen was cryopreserved in 4% dimethyl sulfoxide (DMSO) with betaine supplemented at 0.1, 0.2 and 0.4 M or raffinose supplemented at 1, 5 and 10 mM. Post thaw semen parameters and fertility were evaluated. RESULTS: Betaine at higher concentrations significantly (p < 0.05) inhibited the post thaw sperm motility, live sperm and MTT dye reduction and a declining trend in the fertility with increasing betaine. Inclusion of raffinose had no effect on the post thaw in vitro semen parameters, however, the fertility was significantly (p < 0.05) higher in the 10 mM raffinose supplemented group. CONCLUSION: Betaine has negative effect on post thaw semen parameters and raffinose at 10 mM concentration improves the fertility from cryopreserved semen

Date	2023-07-19T10:03:37Z 2023-07-19T10:03:37Z 2022-5-1

Type	Research Paper

Identifier	— Not Available 0143-2044 http://krishi.icar.gov.in/jspui/handle/123456789/80143

Language	English

Relation	— —

Publisher	Not Available

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