Record Details


Field	Value

Title	Effect of tempol on post-thaw semen parameters and fertility during chicken semen cryopreservation.

Creator	Shanmugam M.

Subject	semen,tempol

Description	Not Available Sperm are exposed to oxidative stress during cryopreservation leading to damage of membrane lipids. In the present study, the effects of adding Tempol during cryopreservation of chicken semen on post-thaw semen parameters and fertility were investigated. Adult PD-1linesemen were cryopreserved using 4% dimethyl sulfoxide (DMSO) in Sasaki diluent (SD). Tempol (1 and 5 mM) was added to the semen-cryopreservation mixture at final concentrations. The semen with additives was filled in 0.5 mL French straws and exposed to liquid nitrogen vapours for 30 min and then stored in liquid nitrogen. The semen straw was thawed at 5 ?C for 100 seconds and evaluated for sperm motility, live, abnormal, and acrosome intact sperm. The semi-nal plasma was evaluated for lipid peroxidation. The fertilization potential of the cryopreserved sperm was evaluated after insemination in the PD-1line hens. Sperm parameters after thawing were significantly (P

Date	2023-07-19T10:03:49Z 2023-07-19T10:03:49Z 2023-1-1

Type	Research Paper

Identifier	— Not Available 2251-628X http://krishi.icar.gov.in/jspui/handle/123456789/80145

Language	English

Relation	— —

Publisher	Not Available

ICAR Research Data Repository for Knowledge Management