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Effects of calorie restriction on the expression of managanese superoxide dismutase and catalase under oxidative stress conditions in the rotifer Brachionus plicatilis.

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Title Effects of calorie restriction on the expression of managanese superoxide dismutase and catalase under oxidative stress conditions in the rotifer Brachionus plicatilis.
Not Available
 
Creator M. Kailasam,M..
Kaneko,G
A.K.S., OOO
Ozaki,Y
Thirunavukkarasu,A.R.
Watabe,S.
 
Subject Brachionus plicatilis
Calorie restriction
Catalase
Juglone
Manganese superoxide dismutase
Oxidative stress
Reactive oxygen species
 
Description Not Available
Calorie restriction (CR) in the rotifer Brachi onus plicatilis extends its lifespan, as it enhances the
expression of antioxidant enzymes such as manganese
superoxide dismutase (Mn SOD) and catalase. Here we
show that CR also increased the mRNA levels of these
antioxidant enzymes upon exposure to oxidative stress.
Rotifers cultured under CR showed a higher survival rate
than those fed ad libitum (AL) upon exposure to
0.05–0.2 lM juglone, an oxidative stress inducer. The
relative mRNA levels of Mn SOD and catalase before
exposure to juglone were slightly higher in the CR rotifers
than in their AL counterparts, although these differences
were not statistically significant. AL rotifers showed no apparent upregulation of the mRNA levels of these anti oxidant enzymes upon exposure to 0.025 and 0.05 lM
juglone. In contrast, the CR rotifers increased the mRNA
levels of Mn SOD and catalase by up to 5.4-fold and
4.2-fold, respectively, resulting in significant differences
between their levels in AL and CR rotifers under oxidative
stress conditions. Furthermore, the protein level of catalase
was clearly higher in CR than in AL rotifers 6 h after
exposure to oxidative stress. These results suggest that the
upregulation of Mn SOD and catalase genes is involved in
CR-induced resistance to oxidative stress in the rotifer
Not Available
 
Date 2023-07-31T09:00:44Z
2023-07-31T09:00:44Z
2010-01-01
 
Type Journal
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/80367
 
Language English
 
Relation Not Available;
 
Publisher Not Available