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Molecular epidemiology of Mycobactetrium avium subspecies paratuberculosis: IS900 PCR identification and IS1311 polymorphism analysis from ruminants in the Punjab region of India.

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Title Molecular epidemiology of Mycobactetrium avium subspecies paratuberculosis: IS900 PCR identification and IS1311 polymorphism analysis from ruminants in the Punjab region of India.
Not Available
 
Creator Kaur,P
Filia,G
Singh,S.V
Patil,P.K
Ravi Kumar,G.V.P.P.S.
Sandhu,K.S.
 
Subject Johne’s disease
Mycobacterium avium subspecies
paratuberculosis
Ziehl–Neelsen staining
PCR
IS900
IS1311
REA
Molecular epidemiology
 
Description Not Available
Johne’s disease is chronic granulomatous infectious enteritis of animals caused by Mycobac terium avium subspecies paratuberculosis. A total of 153 animals from 19 dairy farms, 2
gaushalas (unproductive-animal rehabilitation centers), 2 goat and 2 sheep farms from dif ferent districts of the Punjab region were selected on the basis of clinical signs of disease. All
samples from cattle (n = 86), buffalo (n = 34), goat (n = 25) and sheep (n = 26) were subjected
to Ziehl–Neelsen staining and DNA extraction by a freeze and thaw method. Ziehl–Neelsen
staining detected 71% samples positive for acid-fast bacilli whereas IS900 PCR detected 55%
positive for Map DNA. IS1311 PCR-REA analysis of IS900 positive samples revealed ‘Bison’
type as the most prevalent (82%) genotype of Map, infecting all domestic ruminants. ‘Cattle’
type was present in a minority of cases (15%) from cattle, buffaloes and goats. This is the
first report of ‘Cattle’ type Map from buffalo and goat species in India
Not Available
 
Date 2023-08-01T09:28:44Z
2023-08-01T09:28:44Z
2010-01-01
 
Type Journal
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/80377
 
Language English
 
Relation Not Available;
 
Publisher Not Available