Molecular epidemiology of Mycobactetrium avium subspecies paratuberculosis: IS900 PCR identification and IS1311 polymorphism analysis from ruminants in the Punjab region of India.
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Title |
Molecular epidemiology of Mycobactetrium avium subspecies paratuberculosis: IS900 PCR identification and IS1311 polymorphism analysis from ruminants in the Punjab region of India.
Not Available |
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Creator |
Kaur,P
Filia,G Singh,S.V Patil,P.K Ravi Kumar,G.V.P.P.S. Sandhu,K.S. |
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Subject |
Johne’s disease
Mycobacterium avium subspecies paratuberculosis Ziehl–Neelsen staining PCR IS900 IS1311 REA Molecular epidemiology |
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Description |
Not Available
Johne’s disease is chronic granulomatous infectious enteritis of animals caused by Mycobac terium avium subspecies paratuberculosis. A total of 153 animals from 19 dairy farms, 2 gaushalas (unproductive-animal rehabilitation centers), 2 goat and 2 sheep farms from dif ferent districts of the Punjab region were selected on the basis of clinical signs of disease. All samples from cattle (n = 86), buffalo (n = 34), goat (n = 25) and sheep (n = 26) were subjected to Ziehl–Neelsen staining and DNA extraction by a freeze and thaw method. Ziehl–Neelsen staining detected 71% samples positive for acid-fast bacilli whereas IS900 PCR detected 55% positive for Map DNA. IS1311 PCR-REA analysis of IS900 positive samples revealed ‘Bison’ type as the most prevalent (82%) genotype of Map, infecting all domestic ruminants. ‘Cattle’ type was present in a minority of cases (15%) from cattle, buffaloes and goats. This is the first report of ‘Cattle’ type Map from buffalo and goat species in India Not Available |
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Date |
2023-08-01T09:28:44Z
2023-08-01T09:28:44Z 2010-01-01 |
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Type |
Journal
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Identifier |
Not Available
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/80377 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Not Available
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