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Detection and diversity of nitrifying and denitrifying functional genes in coastal aquaculture.

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Title Detection and diversity of nitrifying and denitrifying functional genes in coastal aquaculture.
Not Available
 
Creator K.K. Krishnani
 
Subject Diversity
Functional genes
Ammonia monooxygenase
Nitrite-oxido-reductase
Nitrite reductase
Nitrous oxide
reductase
Aquaculture
 
Description Not Available
Nucleic acid methods based on sequencing of clone libraries provide sequence and the phylogenetic
information of an individual clone. In the present study, ammonia monooxygenase (amoA), nitrite-oxido reductase(norB), nitrite reductase (nirS) and nitrous oxide reductase (nosZ) genes are chosen to detect nitrifiers
and denitrifiers in coastal aquaculture using gene-specific primers. The abundance of these functional genes
revealed the presence of nitrifying and denitrifying organisms in coastal aquaculture. Nitrifying and
denitrifying communities were analyzed by parallel DNA extractions and clone library construction for amoA,
norB, nirS and nosZ obtained from coastal aquaculture soil. Amino acids in parts of the amoA, norB, nirS and
nosZ encoded proteins were aligned to know conserved amino acid residues. The amoA genes exhibited 81–
82% identity to Nitrosomonas europaea, Nitrosococcus mobilis and Nitrosomonas eutropha which were also
similar to particulate methane monooxygenase (pmoA) gene sequences. The norB genes are closely affiliated
with Nitrobacter winogradskyi and other uncultured beta-proteobacteria. The nosZ genes exhibited 78–82%
identity with Marinobacter sp. and other uncultured denitrifying gamma-proteobacteria. The present study
could be useful for making bioremediation strategy for nitrogenous metabolites and understanding of
nitrogen fluxes generated through these two functional groups in coastal shrimp aquaculture
Not Available
 
Date 2023-08-02T06:33:55Z
2023-08-02T06:33:55Z
2009-01-01
 
Type Journal
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/80388
 
Language English
 
Relation Not Available;
 
Publisher Not Available