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Identification of genic SSRs in jute (Corchorus capsularis, Malvaceae) and development of markers for phenylpropanoid biosynthesis genes and regulatory genes

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Title Identification of genic SSRs in jute (Corchorus capsularis, Malvaceae) and development of markers for phenylpropanoid biosynthesis genes and regulatory genes
Not Available
 
Creator P. Satya, A. Chakraborty, S. Jana, S. Majumdar, M. Karan, D. Sarkar, S. Datta, J. Mitra, C. S. Kar, P. G. Karmakar, N. K. Singh
 
Subject Genic SSRs
Jute
Phenylpropanoids
Transcription factors
Transcriptome
 
Description Not Available
In jute (Corchorus spp.), phenylpropanoid biosynthesis pathway is associated with
lignocellulosic bast fibre development and production of various phytochemicals.
Development of phenylpropanoid-related genic markers is thus a key component of
gene-specific precision molecular breeding in jute. We identified a total of 12,772
SSRs in 10,041 unigenes and designed genic SSR markers associated with
phenylpropanoid biosynthesis from bast transcriptome of Corchorus capsularis cv.
‘JRC-212’. A total of 39 and 14 SSR markers were developed from key genes of
phenylpropanoid biosynthesis and bast fibre formation, respectively. Further, we
developed 457 SSR markers associated with different regulatory gene sequences.
Of these, 137 markers were for transcription factors such as WRKY, MYB, MYBrelated,
bHLH and zinc finger, which are key regulators and/or modulators of the
biosynthesis of phenylpropanoids. Additionally, 600 SSR primer pairs were also
designed for other unigenes containing SSR. Diversity analysis based on a set of 41
genic SSR markers in 35 genotypes revealed high polymorphism. The genic SSRs
described here are expected to be useful for molecular breeding targeting fibre
quality, phytochemical-related and stress tolerance traits in jute.
ICAR Network Project on Transgenics in Crops (ICAR-NPTC)/ ICAR-NPTC-3052/ ICAR-NPTC-3070
 
Date 2018-11-19T09:34:40Z
2018-11-19T09:34:40Z
2017-10-01
 
Type Research Paper
 
Identifier Not Available
1439-0523
http://krishi.icar.gov.in/jspui/handle/123456789/12068
 
Language English
 
Relation Not Available;
 
Publisher Wiley