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Determination of fatty acids and proteins from the fresh water alga Chlamydomonas reinhardtii cc 2137 and its antagonism against aquatic bacteria.

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Title Determination of fatty acids and proteins from the fresh water alga Chlamydomonas reinhardtii cc 2137 and its antagonism against aquatic bacteria.
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Creator S.Kannappan
A.Kumar
R.Sessler
R.T.Sayre
V.Falcoa
U.Ihemere
J.Ndunguru
N. Narayanan,N.
 
Subject Algal strain and medium
Revival of Chlamydomonas reinhardtii
Extraction of cell wall bound protein
Solvent extraction
Total fat extraction
Description of GCMS & fatty acids analysis
Extraction of FAE1 protein from CC2137
Antimicrobial assay by well diffusion method
Statistical analysis
 
Description Not Available
Chlamydomonas reinhardtii, a wild-type algal strain (CC 2137) was grown on tris acetate phosphate agar (TAP)
medium. The growth curve values showed 2.0 logs till 6 days and declined thereafter. Later, the cells were harvested and
extracted for fat using chloroform and methanol (2:1). The extract was hydrolyzed, lyzed and found to have unsaturated
fatty acids like linolenic, linoleic, oleic acid, palmitoleic acid and saturated fatty acids viz. myristic, palmitic and stearic
acid,using gas chromatography as well as mass spectrometry (GC-MS). Cell wall bound iron containing fatty acid
elongation (FAE1) proteins of CC2137 were extracted and their size (ranging from 25-250 KD) was detected by SDS
PAGE. The FAE1 protein was inhibitory to Bacillus cereus as evidenced by “Agar Spot Assay”. Organic extracts of
CC2137 cells with hot and cold extraction were tested against marine bacteria. In cold extraction, methanolic extracts
showed highest inhibition (11.0 mm) to Vibrio harveyi (MM30) and subsequently acetone and ethyl acetate extracts showed
inhibition to V. harveyi (MM32). In hot extraction, highest inhibitions were observed by ethanol and ethyl acetate (13.0
mm) to MM30 followed by methanol, ethanol and ethyl acetate extracts (12.0 mm) to MM32. Extracts of ethyl acetate and
acetic acid showed maximum and identical inhibitions (8.0 mm) against Pseudomonas putida (PP) and lowest by
methanoles and chloroform (6.0 mm) in cold extraction. Under hot extraction, the highest inhibition was shown by ethanol
extract to PP followed by methanol and acetone (11.0 mm) and lowest by chloroform and acetic acid extracts (6.0 mm)
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Date 2023-08-24T05:05:11Z
2023-08-24T05:05:11Z
2012-01-01
 
Type Journal
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/80496
 
Language English
 
Relation Not Available;
 
Publisher Not Available