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Detection of quorum sensing molecules from Vibrio harveyi and use of synthetic furanone to control V.harveyi during shrimp larviculture.

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Title Detection of quorum sensing molecules from Vibrio harveyi and use of synthetic furanone to control V.harveyi during shrimp larviculture.
Not Available
 
Creator S.Kannappan
A.Kumar
I.Uzo
F.Vanessa
N.Narayanan
G.Elisa
R.T. Sayre
 
Subject Vibrio harveyi
Detection of N-aceyl homoserine lactone compounds (AHL)
Furanone
Antagonism
Shrimp larviculture.
 
Description Not Available
This paper explores the extraction and detection processes of quorum sensing molecules such as N aceyl homoserine lactone compounds (AHL) from marine Vibrio harveyi. The spent culture of V. harveyi
was solvent partitioned for AHL, rotary evaporated and re-suspended in 50% acetonitrile then detected
with reporter strain using Luminometer. Furanone was tested for antagonism against V. harveyi and an
inhibitory zone of 9.0 ± 0.25 mm was observed. When, V. harveyi grew in Lysogeny broth (LB) with
furanone, the growth was decreased from 0.2 to 0.03 optical density (OD) in 16 h. In control, V. harveyi
growth was increased from 1.33 to 2.3 OD. When furanone was challenged against V. harveyi among
shrimp post larvae, the cumulative percentage mortality was increased in the control from 0.85 to and
80% for the period of 5th to 30th days. But in the treatment tank the mortality varied from 0.53 to 1.26,
5.51, 8.17, 10.72 and 13.37% till the 30th day. The differences in the cumulative percentage mortalities in
the treatment tank were 0.32, 2.34, 28.63, 51.50, 59.20 and 66.71% respectively as compared to the
control. The results suggest that furanone can be used as non-antibiotic agent to control shrimp
disease caused by V. harveyi
Not Available
 
Date 2023-08-24T05:05:43Z
2023-08-24T05:05:43Z
2012-07-30
 
Type Journal
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/80497
 
Language English
 
Relation Not Available;
 
Publisher Not Available