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Genomic classification of betanodavirus by molecular phylogenetic analysis of the coat protein gene.

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Title Genomic classification of betanodavirus by molecular phylogenetic analysis of the coat protein gene.
Not Available
 
Creator C.P.Binesh
C.Greeshma
K.P.Jithendran
 
Subject Genomic classification
Betanodavirus
Molecular phylogenetic analysis
Coat protein gene.
 
Description Not Available
The classification of betanodavirus into four
species was reviewed including newer and well-charac terised isolates. Six major clusters were identified, four of
which were similar to the classic species. Two single iso late clusters were worth consideration as new species.
Betanodaviruses are small (37 nm average) icosahedral
viruses with two positive-stranded non-polyadenylated
RNAs as its genome. RNA1 (3.1 kb) codes for the viral
RNA-dependent RNA polymerase (protein A), while
RNA2 (1.4 kb) codes for the coat protein [16]. A subge nomic RNA3 synthesized from RNA1 during early viral
replication codes for protein B2 [17], which functions
against the host RNA interference system [3, 8]. These
viruses cause viral nervous necrosis (VNN) or viral
encephalopathy and retinopathy (VER) in fin fishes. They
were first identified as picorna-like virus particles [5] and
later described as members of the family Nodaviridae [12].
Taxonomically, these viruses belong to the genus Betan odavirus of family Nodaviridae, with four species recog nised so far [16] based on similarities in the variable region
of the viral coat protein (CP) gene (nt 604-1030) [14]. The
currently recognized species are Redspotted grouper ner vous necrosis virus (RGNNV), Striped jack nervous
necrosis virus (SJNNV), Tiger puffer nervous necrosis
virus (TPNNV) and Barfin flounder nervous necrosis virus. (BFNNV), with SJNNV as the type species. The preferred
temperature ranges of members of these species are
25-30 C, 20-25 C, 20 C and 15-20 C, respectively.
Over 40 fish species have been reported to be suscep tible to betanodaviruses, and the number is steadily
increasing [10, 13]. Since the first description 15 years ago,
the taxonomy of betanodavirus has remained more or less
the same irrespective of the huge number of virus isolates
being reported from different geographical environments
and from different fish hosts. Some modifications were
proposed to the system [4, 11, 15, 19], but these have been
largely restricted to the geographical area of the
researcher. The study presented here is an attempt to
analyze the global phylogenetic relationships among the
viruses by comparing all isolates for which full-length
coat protein (CP) gene ORF sequence information is
available in the NCBI GenBank database. A short variable
region in the CP gene is shown to be sufficient for
determining the phylogenetics of these viruses [14], but
the full-length CP gene ORF is used in this study to obtain
more reliable results.
Not Available
 
Date 2023-08-24T05:08:42Z
2023-08-24T05:08:42Z
2012-11-10
 
Type Journal
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/80502
 
Language English
 
Relation Not Available;
 
Publisher Not Available