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A new blocking ELISA for detection of foot-and-mouth disease non-structural protein (NSP) antibodies in a broad host range.

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Title A new blocking ELISA for detection of foot-and-mouth disease non-structural protein (NSP) antibodies in a broad host range.
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Creator Hosamani M, Gopinath S, Sreenivasa BP, Behera S, Basagoudanavar SH, Boora A, Bora DP, Deka P, Bhanuprakash V, Singh RK, Sanyal A, Weerdmeester K, Dekker A.
 
Subject Blocking ELISA; Foot-and-mouth disease; Monoclonal antibody; Non-structural protein antibodies.
 
Description Not Available
Large-scale monitoring of foot-and-mouth disease (FMD) in livestock is imperative in an FMD control program. Detection of antibodies against non-structural proteins (NSP) of FMD virus (FMDV) is one of the best tools to estimate the prevalence of past infection; availability of such a well-validated test is therefore essential. Using a FMDV 3B protein-specific monoclonal antibody, we have developed a new NSP antibody blocking ELISA (10H9 bELISA) and validated it on large panels of sera from different susceptible species. The diagnostic sensitivity of the ELISA was 95% with a specificity of 98%, similar to the values found using a commercial kit (PrioCHECK FMD NS test). The 10H9 bELISA can be used in a broad range of FMD susceptible species making it a very useful tool in monitoring the foot-and-mouth disease control programs by detection of virus circulation in the vaccinated populations. KEY POINTS: • A new ELISA for detection of foot and mouth disease (FMD) antibodies. • Diagnostic sensitivity of 95% and specificity of 98%. • Tested with panels of validated sera from broad host range.
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Date 2023-05-12T05:04:56Z
2023-05-12T05:04:56Z
2022-09-22
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/77186
 
Language English
 
Relation Not Available;
 
Publisher Springer