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Study on Plasmid-borne Traits in Xanthomonas campestris pv. viticola Causing Bacterial Leaf Spot in Grapes.

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Title Study on Plasmid-borne Traits in Xanthomonas campestris pv. viticola Causing Bacterial Leaf Spot in Grapes.
Not Available
 
Creator Ashlesha C. Patil, Sujoy Saha, Indu S. Sawant, Sanjay D. Sawant, Ratna U. Thosar. 2022
 
Subject Plasmid, Curing, Antibiotics, Pathogenicity, Xanthomonas campestris pv viticola
 
Description Not Available
Plasmid has been proved an invaluable tool to microbiologist and molecular biologist as they confer a
selective and important advantage to the organisms
that possess them. This study present the first report
on isolation and identification of plasmid from bacteria Xanthomonas campestris pv viticola which is
known to cause bacterial leaf spot disease on grape.
Plasmid was isolated from bacteria followed by its
curing using different curing agents. Pathogenicity,
exopolysacchride production and antibiotic resistance
tests were performed with cured and uncured bacteria
to check virulence and antibiotic sensitivity. Approximately, 23.1 kb size of plasmid was isolated from
bacteria followed by curing it with three different
curing agents viz., sodium dodecyl sulphate, ethidium bromide and acridine orange. Plasmid curing
exhibited negative impact on colony morphology
and exopolysacchride production. Cured bacteria
was also found sensitive to the tested antibiotics. It
also lost its virulence upon artificial inoculation on
different varieties of grapes. Plasmid curing work
indicate that colony morphology, exopolysacchride
production, antibiotic sensitivity and virulence are
plasmid-borne traits.
Not Available
 
Date 2023-12-13T15:11:42Z
2023-12-13T15:11:42Z
2022-07-15
 
Type Research Paper
 
Identifier Not Available
0970-0420
http://krishi.icar.gov.in/jspui/handle/123456789/80967
 
Language English
 
Relation Not Available;
 
Publisher Not Available