Study on Plasmid-borne Traits in Xanthomonas campestris pv. viticola Causing Bacterial Leaf Spot in Grapes.
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Title |
Study on Plasmid-borne Traits in Xanthomonas campestris pv. viticola Causing Bacterial Leaf Spot in Grapes.
Not Available |
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Creator |
Ashlesha C. Patil, Sujoy Saha, Indu S. Sawant, Sanjay D. Sawant, Ratna U. Thosar. 2022
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Subject |
Plasmid, Curing, Antibiotics, Pathogenicity, Xanthomonas campestris pv viticola
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Description |
Not Available
Plasmid has been proved an invaluable tool to microbiologist and molecular biologist as they confer a selective and important advantage to the organisms that possess them. This study present the first report on isolation and identification of plasmid from bacteria Xanthomonas campestris pv viticola which is known to cause bacterial leaf spot disease on grape. Plasmid was isolated from bacteria followed by its curing using different curing agents. Pathogenicity, exopolysacchride production and antibiotic resistance tests were performed with cured and uncured bacteria to check virulence and antibiotic sensitivity. Approximately, 23.1 kb size of plasmid was isolated from bacteria followed by curing it with three different curing agents viz., sodium dodecyl sulphate, ethidium bromide and acridine orange. Plasmid curing exhibited negative impact on colony morphology and exopolysacchride production. Cured bacteria was also found sensitive to the tested antibiotics. It also lost its virulence upon artificial inoculation on different varieties of grapes. Plasmid curing work indicate that colony morphology, exopolysacchride production, antibiotic sensitivity and virulence are plasmid-borne traits. Not Available |
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Date |
2023-12-13T15:11:42Z
2023-12-13T15:11:42Z 2022-07-15 |
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Type |
Research Paper
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Identifier |
Not Available
0970-0420 http://krishi.icar.gov.in/jspui/handle/123456789/80967 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Not Available
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