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Exploring broilers and native fowls of Andaman and Nicobar Islands as a source of b-lactamase-producing Enterobacteriaceae even with limited anthropogenic activities and docking-based identification of catalytic domains in novel b-lactamase variants

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Title Exploring broilers and native fowls of Andaman and Nicobar Islands as a source of b-lactamase-producing Enterobacteriaceae even with limited anthropogenic activities and docking-based identification of catalytic domains in novel b-lactamase variants
 
Creator Sneha Bhowmick
Jai Sunder
Surajit Pal
T. Sujatha
I Samantha
T K Dutta
A K De
T Mondal
A D Singh
S Bandyopadhyay
A K Tiwari
 
Subject Andaman and Nicobar, docking, clonal, ESBL, poultry
 
Description Not Available
The present study was conducted to detect the occurrence of b-
lactamase and biofilm-producing Escherichia coli, Salmonella, and Klebsiella
in broilers and native fowl reared in the Andaman and Nicobar Islands, India.
The study also included molecular docking experiments to confirm the nature
of the catalytic domains found in the b-lactamase variants obtained and to
reveal the clonal relationship of the isolates with human clinical strains from
the database.bleMaterials and methods: A total of 199 cloacal swabs were collected from
five poultry breeds/varieties (broiler, Vanraja, Desi, Nicobari, and layer) in three
districts of the Andaman and Nicobar Islands. E. coli, Salmonella enterica, and
Klebsiella pneumoniae were isolated by standard techniques and confirmed
by PCR. Phenotypical b-lactamase producers were identified by a double-disc
test. The genes (blaCTX, blaSHV, blaTEM, and blaAmpC) were screened,
and selected sequences of b-lactamase variants were submitted to DDBJ.Homologymodeling,model validation, and active site identification of di􀀀erent
b-lactamase variants were done by the SWISS-MODEL. Molecular docking
was performed to identify the catalytic domains of the b-lactamase variants.
The selected b-lactamase sequences were compared with the Indian ESBL
sequences from human clinical strains in NCBI-GenBank.
Results: In total, 425 Enterobacteriaceae strains were isolated from the
collected samples. Klebsiella pneumoniae (42.58%) was found to be the most
prevalent, followed by Salmonella enterica (30.82%) and E. coli (26.58%). The
phenotypical antibiogram of all 425 isolates showed the highest resistance
against oxytetracycline (61–76%) and the lowest against gentamicin (15–
20%). Phenotypical production of b-lactamase enzymes was observed in
141 (33.38%) isolates. The isolation rate of b-lactamase producing E. coli,
Salmonella enterica, and Klebsiella pneumoniae was significantly higher (p
Not Available
 
Date 2023-12-15T15:14:41Z
2023-12-15T15:14:41Z
2023-01-05
 
Type Book
 
Identifier Bhowmick S, Pal S, Sunder J, Sujatha T, De AK, Mondal T, Singh AD, Joardar SN, Batabyal K, Dutta TK, Bandyopadhyay S, Tiwari A and Samanta I (2023) Exploring broilers and native fowls of Andaman and Nicobar Islands as a source of b-lactamase-producing Enterobacteriaceae even with limited anthropogenic activities and docking-based identification of catalytic domains in novel b-lactamase variants. Front. Vet. Sci. 9:1075133. doi: 10.3389/fvets.2022.1075133vailable
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/80996
 
Language English
 
Relation Not Available;
 
Publisher Not Available