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Agroclimatic zone-wise prevalence of biofilm forming methicillin resistant staphylococcus aureus in West Bengal

Indian Agricultural Research Journals

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Title Agroclimatic zone-wise prevalence of biofilm forming methicillin resistant staphylococcus aureus in West Bengal
 
Creator BARDHAN, RAJARSHI
PAUL, ADITYA
SAMANTA, INDRANIL
BATABYAL, KUNAL
DEY, SAMIR
SARKAR, UTTAM
JOARDAR, SIDDHARTHA NARAYAN
 
Subject Agro-climatic zones, Biofilm, Cattle, Milk, Methicillin resistance
 
Description The present study envisages the prevalence of biofilm producing, methicillin resistant Staphylococcus aureus, isolated from cattle milk of different agro-climatic zones of an eastern state of India, West Bengal. The milk samples (n=168) were collected from three predominant agro- climatic zones of South Bengal, viz. new alluvial, coastal saline and red laterite. A total of 146 Staphylococcus aureus isolates were recovered, confirmed by biochemical tests and polymerase chain reaction (PCR). Through PCR of mecA gene, it was found that 45 strains were methicillin resistant, encompassing 28.57% new alluvial zone, 30.19% red laterite region and 29.16% coastal saline region. Determination of biofilm formation was done, both phenotypically by microtiter plate method and genotypically by PCR of icaA and icaD genes, specific for biofilm formation in Staphylococcus aureus. Phenotypic study revealed that 14 isolates were strong biofilm producers, 79 were moderate and the 47 were weak biofilm producers. PCR studies also showed that a total of 30 isolates were icaA positive and 25 isolates were icaD positive. Although no significant difference was found in the occurrence of icaA positive isolates, the possession of icaD by the Staphylococcus aureus isolates varied significantly according to different agro-climatic zones.
 
Publisher Indian Council of Agricultural Research
 
Date 2024-08-06
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
application/pdf
 
Identifier https://epubs.icar.org.in/index.php/IJAnS/article/view/147438
10.56093/ijans.v94i8.147438
 
Source The Indian Journal of Animal Sciences; Vol. 94 No. 8 (2024); 681–685
2394-3327
0367-8318
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/IJAnS/article/view/147438/55360
https://epubs.icar.org.in/index.php/IJAnS/article/view/147438/55361
 
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