Ultrastructure of sperm of the Spotted scat (Scatophagus argus, Linnaeus, 1766) observed by scanning and transmission electron microscopy

Abstract

An investigation was conducted to understand the sperm cell morphology and ultrastructure of Spotted scat (Scatophagus argus) through scanning and transmission electron microscopy. The present study reveals that the sperm of S. argus can be differentiated into three major parts – an acrosome-less spherical head, a short mid-piece, and a cylindrical flagellum. The scat sperm cell had a mean total length of 21.32±1.80 mwith the presence of ovoid electron-dense nucleus. The mean length and width of ovoid nucleus measured 1.44±0.34 and 1.54±0.33 m, respectively. The structural characteristics of the nucleus were found to be a shallow axial nuclear fossa and centriolar complex. The two centrioles were positioned nearly perpendicular to each other with a conventional “9 + 0” pattern in the proximal centriole. The short mid-piece was located laterally to the nucleus and contains 5 or 6 spherical and unequal-sized mitochondria. The mitochondria were separated from the axoneme by a cytoplasmic canal. The flagellum was inserted at the base of the nucleus with the presence of an axoneme structure of 9 + 2 paired micro tubules. The sperm flagellum had short irregular lateral fins. The present study reveals that Spotted scat sperm can be categorized as being of a “primitive or ect-aquasperm type” and belongs to the teleostean “type I” sperm. This is the first report on the morphology and ultrastructure of sperm in Scatophagidae family.