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http://krishi.icar.gov.in/jspui/handle/123456789/10897| Title: | RAPD and ISSR markers for molecular characterization of Grewiaoptiva: an important fodder tree of north western Himalayas |
| Other Titles: | Not Available |
| Authors: | Not Available |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | Dr . Y . S. Parmar University of Horticulture and Forestry , Nauni-Solan-173 230, Himachal Pradesh |
| Published/ Complete Date: | 2015-08-01 |
| Project Code: | Not Available |
| Keywords: | Fodder tree, Genetic diversity,Grewia optiva, ISSR, RAPD |
| Publisher: | Not Available |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | RAPD (random amplified polymorphic DNA) and ISSR (inter simple sequence repeats) markers were used to study DNA polymorphism of thirty Grewia optivagenotypes raised from seeds collected from various districts of Himachal Pradesh (India) and selected on the basis of morphological parameters. In general, the genotypes exhibited a very high level of molecular diversity and DNA polymorphism. Using 25 RAPD and 18 ISSR primers molecular diversity based on Jaccard’s similarity coefficients among 30 genotypes ranged from 0.17-0.83 in RAPD assay and 0.30-0.84 in ISSR assay. RAPD primers amplified more loci than ISSR primers. RAPD and ISSR primers amplified 96.31 and 91.72% polymorphic loci, respectively. The dendrogram derived using the unweighted pair-group method with arithmetic averaging (UPGMA) clustered the genotypes into different groups reflecting geographical sub-structuring of the genetic diversity. Genotype SO-12 (Kasauli) was found to be the most divergent genotype which could be used for number of combinations to be established in the seed orchards and as a parent in hybridization programme |
| Description: | Not Available |
| ISSN: | 0971-2070 |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Range Management and Agroforestry |
| NAAS Rating: | 6.28 |
| Volume No.: | 36 |
| Page Number: | 26-32 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | Not Available |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/10897 |
| Appears in Collections: | NRM-CAZRI-Publication |
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