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http://krishi.icar.gov.in/jspui/handle/123456789/13282| Title: | A novel bio-engineering approach to generate an eminent surfacefunctionalized template for selective detection of female sex pheromone of Helicoverpa armigera |
| Authors: | Parikshit Moitra Deepa Bhagat Rudra Pratap Santanu Bhattacharya |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | Department of Organic Chemistry, Indian Institute of Science, Bangalore 560 012, India National Bureau of Agriculturally Important Insects, Indian Council of Agricultural Research, Bangalore 560 024, India Centre of Nano Science and Engineering, Indian Institute of Science, Bangalore 560 012, India Department of Organic Chemistry, Indian Institute of Science, Bangalore 560 012, India J. C. Bose Fellow, Department of Science and Technology, New Delhi Also at JNCASR, Bangalore 560 012, India |
| Published/ Complete Date: | 2016-11-28 |
| Project Code: | OXX03331 |
| Keywords: | Agroecology, Biosurfaces, Chemical Engineering |
| Publisher: | Nature Publishing Group |
| Citation: | 4 |
| Abstract/Description: | Plant pests exert serious effects on food production due to which the global crop yields are reduced by ~20–40 percent per year. Hence to meet the world’s food needs, loses of food due to crop pests must be reduced. Herein the silicon dioxide based MEMS devices are covalently functionalized for robust and efficient optical sensing of the female sex pheromones of the pests like Helicoverpa armigera for the first time in literature. The functionalized devices are also capable of selectively measuring the concentration of this pheromone at femtogram level which is much below the concentration of pheromone at the time of pest infestation in an agricultural field. Experiments are also performed in a confined region in the presence of male and female pests and tomato plants which directly mimics the real environmental conditions. Again the reversible use and absolutely trouble free transportation of these pheromone nanosensors heightens their potentials for commercial use. Overall, a novel and unique approach for the selective and reversible sensing of female sex pheromones of certain hazardous pests is reported herein which may be efficiently and economically carried forward from the research laboratory to the agricultural field. |
| Description: | we introduce a novel cost-efficient pheromone sensing based pest detection that uses a microelectromechanical system (MEMS) device15–30 especially designed and functionalized for this purpose. We have developed a MEMS sensor using microcantilevers and fixed-fixed beams as resonant mass sensors for the selective detection of female sex pheromone of Helicoverpa armigera (Hubner), lepidopterous pest of cotton, tomato, rice, pigeonpea and chickpea etc., in air. To the best of our knowledge, this is the first study where silicon dioxide based MEMS devices are covalently functionalized to selectively detect the pheromone molecules for specific insects with sensitivity upto femtogram (fg) level. The successful functionalization of the substrate has been verified by various analytical techniques such as atomic force microscopy (AFM), laser doppler vibrometry (LDV), X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry (ToF-SIMS). The relative sensitivity of these sensors was further improved by different functionalization protocols which increased the number of amine functionalities at each anchor site. The detection limit was observed to be 4.06 ± 0.5 fg of pheromone mass attached to the functionalized cantilevers, which is well below the concentration found for the pheromone at the time of infestation as per the OECD Monograph Guidance – Pheromones and Semiochemicals - September 200231. These MEMS sensors not only eliminate the use of bulky, complex, and fragile instruments, but also reduce the cost considerably for large-scale sensor deployment in real field. Further these pheromone sensors show excellent efficacy and stability in the open atmosphere, even during wet atmospheric conditions like rainy season or in peak summer under bright sunshine. This feature together with the trouble-free transportation aspect of this reversible sensor heightens their potential for the commercial use in any season under ambient conditions. In addition, the chemical functionalizations of the devices have photochemical and thermal stability. The functionalized MEMS devices are also capable of estimating the pheromone concentration present in a field or vineyard and thus may help in determining the level of infestation. The recognition of the pheromone molecules even before visual onset may alert the farmers to take necessary actions in a localized manner before crop loss starts to occur. Hence this approach may be efficiently and economically used in agricultural farms to significantly reduce crop losses and the attendant financial losses. |
| ISSN: | 2045-2322 |
| Type(s) of content: | Journal |
| Sponsors: | Indian Institute of Science, Bangalore |
| Language: | English |
| Name of Journal: | Scientific Reports |
| NAAS Rating: | 10 |
| Volume No.: | 6 |
| Page Number: | 37355 |
| Name of the Division/Regional Station: | Department of Organic Chemistry |
| Source, DOI or any other URL: | https://doi.org/10.1038/srep37355 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/13282 |
| Appears in Collections: | CS-NBAIR-Publication |
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