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http://krishi.icar.gov.in/jspui/handle/123456789/14074
Title: | Identification of Resistance Gene Analogs (RGAS) from Highly Wilt Resistant Castor (Ricinus Communis L.) Genotype |
Other Titles: | Not Available |
Authors: | Kapadia C. V., Mahatma M. K., Parekh M. J., Patel Nafisa and Tomar R. S. |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR-Directorate of Groundnut Research |
Published/ Complete Date: | 2015-03-28 |
Project Code: | Not Available |
Keywords: | Castor, Fusarium oxysporium, NBS-LRR, R-gene, wilt. |
Publisher: | Research Journal of Biotechnology |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | Castor (Ricinus communis) is highly susceptible to bacterial and fungal pathogens. The identification of resistance gene analogs holds great promise for development of resistant castor cultivars. Primer selection was performed based on different combinations of motifs conserved in R-gene classes. Degenerate primers designed based on known resistance genes (R-genes) were used in combinations to elucidate resistance gene analogs from Castor. Only seven primer combinations gave amplicons with expected sizes of 450-900 bp. The nucleotide sequence of these amplicons was obtained through sequencing; their predicted amino acid sequences compared to each other. Only two sequences, namely p6.4 and 23 had significant sequence identity with known R-genes sequences of other plants. The findings of conserved domains, viz., kinase-1a, kinase-2 and hydrophobic motif, provided evidence that the sequences belong to the NBS-LRR class gene family. Homology and % identity searches of the isolated RGA sequences with the NCBI GenBank database showed that p6.4 had 89% identity to the disease resistance like proteins in castor and 23 had 100% identity with I2C disease resistant proteins in tomato. Phylogenetic analysis indicated that the RGAs clustered with R genes found in Arabidopsis and Tomato. The findings will be useful for studying of resistance mechanism in castor to Fusarium oxysporium. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Research Journal of Biotechnology |
NAAS Rating: | 4 |
Volume No.: | 10(5) |
Page Number: | 16-26 |
Name of the Division/Regional Station: | Crop sicence |
Source, DOI or any other URL: | Not Available |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/14074 |
Appears in Collections: | CS-DGR-Publication |
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