The findings of conserved domains, viz., kinase-1a, kinase-2 and hydrophobic motif, provided evidence that the sequences belong to the NBS-LRR class gene family. Homology and % identity searches of the isolated RGA sequences with the NCBI GenBank database showed that p6.4 had 89% identity to the disease resistance like proteins in castor and 23 had 100% identity with I2C disease resistant proteins in tomato. Phylogenetic analysis indicated that the RGAs clustered with R genes found in Arabidopsis and Tomato. The findings will be useful for studying of resistance mechanism in castor to Fusarium oxysporium.

Abstract

Random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) are PCR-based molecular techniques used for evaluation and characterization of date palm to find the best genotype and male/female identification at an early stage. Genetic fingerprinting using molecular markers is an important tool for the analysis of genetic diversity and cultivar identification. Here, we present an improved DNA extraction protocol using leaf tissue, based on the standard cetyltrimethyl ammonium bromide (CTAB) protocol, which yields large amounts of high-quality amplifiable DNA. RAPD and ISSR markers reveal sufficient genetic diversity as well as give some unique markers in some genotypes with a maximum number of bands.