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http://krishi.icar.gov.in/jspui/handle/123456789/15402| Title: | Valorization of Fish Viscera for Crude Proteases Production and Its Use in Bioactive Protein Hydrolysate Preparation |
| Other Titles: | Not Available |
| Authors: | Murthy, L.N. Phadke, G.G. Parvathy U. Jeyakumari, A., Joshy, C.G. Zynudheen, A.A. Ravishankar, C.N. |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Central Institute of Fisheries Technology |
| Published/ Complete Date: | 2017-05-30 |
| Project Code: | Not Available |
| Keywords: | Crude enzyme Tuna visceral waste Croaker protein hydrolysate Response surface methodology Enzyme to substrate ratio Bioactive properties |
| Publisher: | Springer Netherlands |
| Citation: | Murthy, L.N., Phadke, G.G., Parvathy U., Jeyakumari, A., Joshy, C.G., Zynudheen, A.A. and Ravishankar, C.N. (2017) - Valorization of fish viscera for crude proteases production and its use in bioactive protein hydrolysate preparation, Waste & Biomass Valorization, 4: 1-12. |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Increasing environmental pollution and limited biological resources has emphasized the need for better utilization of fish processing waste. In the present study, visceral proteases from little tuna (Euthynnus affinis), catla (Catla catla) and tilapia (Oreochromis mossambicus) belonging to different habitats using acetone, ethanol and ammonium sulfate fractional precipitation were isolated and characterized. Enzymes precipitated from little tuna and tilapia exhibited higher specific activity at 40% saturation for ammonium sulfate fractional precipitation and the specific activities were 18.19 and 13.67 U/mg for little tuna and tilapia enzymes, respectively. Enzymes precipitated from catla reported higher specific activity of 8.32 U/mg at 60% saturation for ammonium sulfate fractional precipitation. Acetone precipitated fraction exhibited higher recovery for all crude enzymes studied. The effect of different combinations of crude tuna visceral waste enzyme and papain enzyme on hydrolysate properties was optimized using response surface methodology. Second order response surface regression model was used to explain the total variability in terms of quadratic and interaction effects of combination enzyme concentration on the properties of croaker protein hydrolysate with a significant R2 value for protein content (0.92), tyrosine content (0.99), degree of hydrolysis (0.99), DPPH free radical scavenging activity (0.97), ferric reducing antioxidant power (0.93), metal chelating activity (0.88), foaming capacity (0.92) and foam stability (0.88). Based on the desirability score, papain at 0.81% concentration and crude tuna visceral protease at 4.36% was found to be optimal for achieving desired properties of croaker protein hydrolysate. |
| Description: | Not Available |
| ISSN: | 1877-2641(Print) 1877-265X (Online) |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Waste and Biomass Valorization |
| NAAS Rating: | 8.85 |
| Volume No.: | 9(10) |
| Page Number: | 1735–1746 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | https://doi.org/10.1007/s12649-017-9962-5 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/15402 |
| Appears in Collections: | FS-CIFT-Publication |
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