Draft Genome Sequence of Bacillus sp. Strain NSP9.1, a Moderately Halophilic Bacterium Isolated from the Salt Marsh of the Great Rann of Kutch, India

Abstract

Bacillus sp. strain NSP9.1 (16S rRNA GenBank accession number JF802181), a moderately halophilic and endosporeforming bacterium, was isolated from the salt marsh of the Great Rann of Kutch, India. It grows optimally at a 7.5% NaCl (range, 0 to 15%) concentration in medium at 37°C and pH 7.5. The genome of Bacillus sp. NSP9.1 was sequenced with a view to understanding the mechanism(s) of osmotolerance and mining the relevant gene(s). The whole genome of NSP9.1 was sequenced using the Roche 454 genome sequencer (GS FLX) at Macrogen Inc., South Korea, through Sequencher Tech Pvt. Ltd., Ahmedabad, India, by both shotgun and mate-paired library sequencing. In shotgun sequencing, an average read length of 412 bp was obtained from 760,964 reads of 314,016,162 bases. Sequencing of mate-pair libraries generated 148,132 reads of 70,321,948 bp and 148,351 reads of 70,918,411 bp, with average read lengths of 474 bp and 478 bp, respectively.