Draft Genome Sequence of the Obligate Halophilic Bacillus sp. Strain NSP22.2, Isolated from a Seasonal Salt Marsh of the Great Rann of Kutch, India

Abstract

The genomes of a number of species of Bacillus, isolated from the extremely saline environments of the Rann of Kutch, India, have been sequenced recently to understand the mechanisms of osmotolerance (1–4). Bacillus sp. strain NSP22.2 (16S rRNA GenBank accession no. JF802172), isolated from a seasonal salt marsh of the Great Rann of Kutch, India, grows optimally at a concentration of 7.0% NaCl (range, 5 to 20%) in growth medium, at pH 7.0 and 37°C. The genome of Bacillus sp. strain NSP22.2 was sequenced to understand its obligate and moderate halophilism and to isolate the relevant gene(s). The Illumina HiSeq 2000 platform was used to sequence the genome of NSP22.2 at Macrogen, Inc., South Korea, through Sequencher Tech Pvt. Ltd., Ahmedabad, India. Originally, a total of 66,480,406 paired-end reads of 6,714,521,006 bases were generated, with a length of 72 nucleotides. After filtering (criteria, 90% of bases with a quality value [QV] of 20 for both ends) and the removal of PCR duplicates, 54,215,856 reads of 5,475,801,456 bases were obtained.