Draft Genome Sequence of Bacillus sp. Strain SB47, an Obligate Extreme Halophile Isolated from a Salt Pan of the Little Rann of Kutch, India

Abstract

Bacillus sp. strain SB47, an obligate extreme halophile and endospore-forming bacterium, was isolated from a salt pan of the Little Rann of Kutch, India. It grows optimally at a 15% NaCl (range, 5 to 35%) concentration in medium at 37°C and at pH 7.5. The genome of Bacillus sp. strain SB47 was sequenced to understand the mechanisms of its extreme osmotolerance and to isolate the relevant gene(s). The whole genome of Bacillus sp. SB47 (G C content of 45.50%) was sequenced using the Roche 454 Genome Sequencer (GS FLX) at Macrogen Inc., South Korea, through Sequencher Tech Pvt. Ltd., Ahmedabad, India, by both shotgun and matepaired library sequencing. In shotgun sequencing, an average read length of 360 bp was generated from 603,934 reads of 217,996,405 bases. Sequencing of the mate-pair libraries gave 151,827 and 131,939 reads, respectively, with average read lengths of 475 bp and 455 bp, respectively.