Draft Genome Sequence of the Extremely Halophilic Bacillus sp. Strain SB49, Isolated from a Salt Crystallizer Pond of the Little Rann of Kutch, India

Abstract

An extremely halophilic and endospore-forming bacterium, Bacillus sp. strain SB49 (16S rRNA GenBank accession number JF802167), was isolated from a salt crystallizer pond of the Little Rann of Kutch, Gujarat, India. This organism grows optimally at 15% NaCl (range, 0 to 30%) concentration in medium at a temperature of 37°C and pH 7.5 (range, 5 to 9). The genome of Bacillus sp. strain SB49 was sequenced to understand the mechanism(s) of extreme osmotolerance and to isolate the relevant gene(s). The genome of Bacillus sp. SB49 was sequenced by shotgun as well as mate-paired libraries at Macrogen Inc., South Korea, through Sequencher Tech Pvt. Ltd., Ahmedabad, India. In shotgun sequencing, an average read length of 453 bp was generated from 845,614 reads of 382,673,464 bases. However, average read lengths of 466 bp and 465 bp, respectively, were generated in mate-pair libraries from 155,818 reads of 72,629,111 bases and 152,361 reads of 70,866,553 bases, respectively.