Draft Genome Sequence of Bacillus sp. Strain NSP2.1, a Nonhalophilic Bacterium Isolated from the Salt Marsh of the Great Rann of Kutch, India

Abstract

The genomes of a number of species of Bacillus inhabiting the Little Rann and Great Rann of Kutch, India, have been sequenced recently with the aim of understanding their mechanism(s) of osmotolerance (1–3). Bacillus sp. strain NSP2.1 (16SrRNA GenBank accession no. JF802192), a nonhalophilic and endospore-forming bacterium isolated from the salt marsh of the Great Rann of Kutch, India, grows optimally without NaCl in the growth medium (NaCl range, 0 to 4.5%), at 37°C, and a pH 7.5. The genome of Bacillus sp. NSP2.1 was sequenced with the aim of understanding its survival mechanism(s) in the salt marsh. The whole genome of NSP2.1 was sequenced by both shotgun and mate-paired library sequencing using the Roche 454 genome sequencer (GS FLX) at Macrogen Inc., South Korea, through Sequencher Tech Pvt. Ltd., Ahmedabad, India. In shotgun sequencing, 769,707 reads of 347,675,293 bases were obtained (average read length, 451 bp). The sequencing of mate-paired libraries generated 140,804 reads of 63,691,257 bp (average read length, 452 bp) and 140,783 reads of 64,402,285 bp (average read length, 457 bp).