Insight into the First Draft Genome Sequence of the Genus Sediminibacillus, Sediminibacillus halophilus Strain NSP9.3

Abstract

In the recent past, genomes of a number of halophiles, thriving in the saline environments of the Rann of Kutch, India, were sequenced to understand the mechanism(s) of osmotolerance (1–4). Sediminibacillus halophilus strain NSP9.3 (16S rRNA, GenBank accession no. JX518263), a moderately halophilic bacterium, was isolated from a seasonal salt marsh of the Great Rann of Kutch, India. This bacterium grows optimally at a 5% NaCl (range, 0 to 20%) concentration in growth medium at 37°C and 7.5 pH. The genome of Sediminibacillus halophilus strain NSP9.3 was sequenced to gain insight into its osmotolerance mechanism( s). The whole genome of Sediminibacillus halophilus strain NSP9.3 (G Ccontent, 44.05%) was sequenced at Macrogen, Inc., South Korea, through Sequencher Tech Pvt., Ltd., Ahmedabad, India, using a Roche 454 genome sequencer (GS FLX), by both shotgun and 3-kb mate-paired library sequencing. Sequencing of shotgun and mate-paired libraries generated 782,771, 137,390, and 122,992 reads of 432,228,177, 60,630,498, and 53,887,735 bases with average read lengths of 552, 441, and 438 bp, respectively.