Draft Genome Sequence of Salinibacillus aidingensis Strain MSP4, an Obligate Halophilic Bacterium Isolated from a Salt Crystallizer of the Rann of Kutch, India

Abstract

Salinibacillus aidingensis strain MSP4 (16S rRNA GenBank accession number JX518262), an obligate halophilic and endospore-forming bacterium, was isolated from a salt crystallizer of the Rann of Kutch, India. It grows optimally at a 10% (range, 8 to 23%) NaCl concentration in medium at a 37°C temperature and 7.0 pH. The genome of Salinibacillus aidingensis MSP4 was sequenced with a view to understanding the echanism(s) of osmotolerance and to mine relevant gene(s). The whole genome of MSP4 was sequenced using the Roche 454 Genome Sequencer (GS FLX). Both shotgun and mate-paired library sequencing were performed at Macrogen Inc., South Korea, through Sequencher Tech Pvt. Ltd., Ahmedabad, India. In shotgun sequencing, 761,949 reads of 343,334,611 bases, with an average read length of 450 bp, were generated. In mate-pair libraries, however, 137,026 and 128,147 reads were produced, respectively, with average read lengths of 443 bp and 452 bp, respectively.